产品名称
抗CD4-FITC小鼠单克隆抗体, clone WM-59, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
WM-59, monoclonal
form
buffered aqueous solution
mol wt
antigen 130-140 kDa
species reactivity
human
technique(s)
indirect immunofluorescence: 1:100 using endothelial cells in human placenta frozen sections
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PECAM1(5175)
General description
识别 CD31 (PECAM-1、endoCAM、gpIIa、hec7)、人细胞表面整合膜糖蛋白。该抗体可增加 TGFβ1在 U937 细胞中诱导的同型聚集率，其与血小板的结合在洗涤后增强。
第 5 次研讨会：代码P025
第 5 次研讨会：代码P025
人 PECAM-1 抗原（血小板内皮细胞粘附分子，CD31，endoCAM。gpⅡa，hec7）是细胞黏附分子免疫球蛋白基因超家族的 130-140kd 单链整合膜糖蛋白成员。人 PECAM-1 (CD31) 表达于血小板、骨髓细胞、B 淋巴细胞、某些 T 淋巴细胞亚群、骨髓前体细胞和 NK 细胞。PECAM 在内皮细胞中大量表达。在培养的内皮细胞单层中，它定位在细胞内连接处。PECAM-1 (CD31) 在嗜同性和嗜异性细胞间粘附并在细胞信号活动中起作用。它在内皮细胞连接处进入内皮下基质中的单核细胞，中性粒细胞和NK细胞的迁移中起主要作用。PECAM-1 (CD31) 可能还参与心血管发育、炎症、血栓形成、创伤愈合和血管生成过程中的一些交互事件。
人CD31（PECAM-1）在血小板、内皮细胞、髓样细胞、B淋巴细胞和某些T淋巴细胞亚群种表达。CD31（PECAM-1）在嗜同性细胞间粘附和嗜异性细胞间粘附以及细胞信号传导活动中起作用。
分化簇31（CD31）也称血小板-内皮细胞粘附分子1（PECAM1）基因，长度为75kb，位于17号人染色体长臂上。该基因编码130kDa蛋白，该蛋白属于免疫球蛋白超家族。它是内皮细胞细胞间连接（ intercellular junction）的重要组成部分。CD31在循环血小板、单核细胞、中性粒细胞和分泌的T淋巴细胞亚群中表达。
Immunogen
人细胞系 RC-2A，最初来源于髓系白血病细胞。
Physical form
0.01米磷酸盐缓冲盐水溶液，pH 7.4
Preparation Note
产品经过 0.2μm 过滤器过滤
Application
小鼠单克隆WM-59抗人PECAM-1 (CD31)可用于研究PECAM-1在细胞间相互作用中的功能，用于血液和组织中 CD31 细胞的检测和计数，以及免疫亲和层析法分离 PECAM-1。单克隆抗人 PECAM-1 (CD31) 显示可增加 TGFβ1在 U937 细胞中诱导的同型聚集率。据报道，其与血小板的结合在洗涤和伴随活化后将增强。
小鼠抗CD31 (PECAM-1) 单克隆抗体可用于免疫组织化学和免疫细胞化学。
Biochem/physiol Actions
分化簇31（CD31）在炎症过程中导致白细胞外渗的粘附级联中起重要作用。该编码蛋白对白细胞通过血管内皮细胞的细胞间连接进行迁移至关重要。该基因的遗传多态性与动脉粥样硬化事件有关。CD31是一种膜糖蛋白，可促进同亲性和异亲性粘附。CD31还参与血管生成。
可通过人细胞表面整合膜糖蛋白识别 CD31（PECAM-1、endoCAM、gpIIa、hec7）。此抗体提高 U937细胞中TGF-b1诱导同型聚集的速率。洗涤后与血小板的反应性增强。
第 5 次研讨会：代码P025
第 5 次研讨会：代码P025
Disclaimer
除非我们的产品目录或产品随附的其他公司文件中另有说明，否则我们的产品仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
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