form
lyophilized powder
extent of labeling
0.1-1.0 mg per mL
matrix
polyacrylhydrazido-agarose
matrix attachment
ribose moiety after periodate oxidation
swelling
1 g swells to ~4 mL
capacity
≥0.2 mg/mL binding capacity (poly(A) (P 9403))
storage temp.
−20°C
Quality Level
Application
Polyuridylic acid-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polyuridylic acid has been used to study insulin receptors and to develop polyclonal antibodies against RNase L.
Physical form
Lyophilized powder stabilized with lactose
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Ling Du et al.
RNA (New York, N.Y.), 11(9), 1340-1347 (2005-07-27)
Activity-dependent changes in protein synthesis modify synaptic efficacy. One mechanism that regulates mRNA translation in the synapto-dendritic compartment is cytoplasmic polyadenylation, a process controlled by CPEB, the cytoplasmic polyadenylation element (CPE)-specific RNA binding protein. In neurons, very few mRNAs are
S H Kim et al.
The EMBO journal, 11(6), 2319-2326 (1992-06-01)
Unlike autocatalyzed self-splicing reactions, nuclear pre-mRNA splicing requires transacting macromolecules and ATP. A protein encoded by the PRP2 gene of Saccharomyces cerevisiae is required, in conjunction with ATP, for the first cleavage-ligation reaction of pre-mRNA splicing. In this study, we
C F Wright et al.
The Journal of biological chemistry, 276(44), 40680-40686 (2001-09-08)
Vaccinia virus gene expression is temporally regulated, and three gene classes have been identified: early, intermediate, and late. Several virus-encoded proteins and an activity designated VLTF-X are required for maximum transcription in vitro of a template containing a late promoter.
E A Zemskov et al.
Journal of virology, 74(15), 6784-6789 (2000-07-11)
The Bombyx mori nucleopolyhedrovirus (BmNPV) genome contains five related members of the bro gene family, all of which are actively expressed in infected BmN cells. Although their functions are unknown, their amino acid sequences contain a motif found in all
Shijun Cheng et al.
The Journal of biological chemistry, 282(35), 25247-25258 (2007-07-04)
The poly(A)-binding protein (PABP), a protein that contains four conserved RNA recognition motifs (RRM1-4) and a C-terminal domain, is expressed throughout the eukaryotic kingdom and promotes translation through physical and functional interactions with eukaryotic initiation factor (eIF) 4G and eIF4B.
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