一般描述
卷心菜磷脂酶D(PLD)包含C2结构域和带HxKx4Dx6GSxN(HKD)基序的PLD超家族特异性保守区域(I–IV)。结构上是一种连接钙离子的桶状单体。
应用
在荧光标记脂质制备过程中，该酶已可用于制备氟硼吡咯-磷脂酰胆碱。
磷脂酶D(PLD)水解磷脂与鞘磷脂的磷酸键，得到相应的磷脂酸。它已可用于研究代谢标记和 体内 直接成像胆碱磷脂，通过测量propargyl-cho的掺入。 此外，PLD已可用于纯化和动力学研究。
生化/生理作用
卷心菜磷脂酶D(PLD)的氨基酸序列与拟南芥PLDα和蓖麻子PLD的一致性分别为83%和80%。 磷脂酶D水解磷脂和鞘磷脂的磷酸键，从而得到相应的磷脂酸。
磷脂酶D（来源于卷心菜）广泛用于生物催化的转磷脂酰反应。
水解磷脂和鞘磷脂的磷酸键，获得相应的磷脂酸。
单位定义
在pH为5.6，温度为30°C的条件下，一个单位酶每小时可从 L-α-磷脂酰胆碱（蛋黄）中释放1.0 μmole的胆碱。
分析说明
蛋白质为双缩脲法测定。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
T T Allgyer et al.
Biochemistry, 18(24), 5348-5353 (1979-11-27)
Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex
Alexandra Lerchner et al.
Protein engineering, design & selection : PEDS, 19(10), 443-452 (2006-07-18)
In addition to hydrolysis of glycerophospholipids, phospholipases D (PLDs) catalyze the head group exchange. The molecular basis of this transphosphatidylation potential, which strongly varies for PLDs from different sources, is unknown hitherto. Recently, the genes of two PLD isoenzymes from
Susanne Stumpe et al.
The FEBS journal, 274(10), 2630-2640 (2007-04-25)
Phospholipases D play an important role in the regulation of cellular processes in plants and mammals. Moreover, they are an essential tool in the synthesis of phospholipids and phospholipid analogs. Knowledge of phospholipase D structures, however, is widely restricted to
Premysl Pejchar et al.
The New phytologist, 188(1), 150-160 (2010-07-16)
• Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the
Cindy Y Jao et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(36), 15332-15337 (2009-08-27)
Choline (Cho)-containing phospholipids are the most abundant phospholipids in cellular membranes and play fundamental structural as well as regulatory roles in cell metabolism and signaling. Although much is known about the biochemistry and metabolism of Cho phospholipids, their cell biology
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