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一般描述
用于真菌和酵母提取物的蛋白酶抑制剂混合液是蛋白酶抑制剂在DMSO溶液中的混合物。该混合液已经过优化和测试，可用于真菌和酵母样品，特别是酿酒酵母细胞。 抑制从20 g酵母中提取的蛋白酶，推荐使用1 mL混合液。
特异性
抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。
应用
Protease Inhibitor Cocktail has been used as a component in:
- FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
- potassium phosphate buffer (pbs) to digest chitin
- extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis
特点和优势
- 广泛的特异性：抑制广泛的蛋白酶，对真菌和酵母提取物提供全面的保护。
- 在酿酒酵母细胞上进行了测试：混合液已针对这种通常研究的酵母菌株进行优化。
- 方便的包装：可采用1或5 mL玻璃瓶，便于处理和储存。
- 即用型溶液：混合液在DMSO溶液中提供，可立即用于蛋白酶抑制试验。
- 有效抑制：混合液中的每种成分都经过精心挑选，以确保其特定的抑制特性，确保可靠和一致的结果。
组分
AEBSF，100 mM
E-64，1.4 mM
抑肽素A，2.2 mM
1,10-菲咯啉，500 mM
E-64，1.4 mM
抑肽素A，2.2 mM
1,10-菲咯啉，500 mM
其他说明
蛋白酶抑制剂混合物，具有抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶的广谱特异性。 含有4-(2-氨基乙基)苯磺酰氟（AEBSF）、抑肽素A、E-64和1,10-菲咯啉。
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
数量
抑制从20 g酵母中提取的蛋白酶，推荐使用1 mL。
外形
DMSO溶液
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产品编号
说明
价格
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2
WGK
WGK 3
闪点(°F)
188.6 °F
闪点(°C)
87 °C
法规信息
常规特殊物品
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Molecular & cellular proteomics : MCP, 10(7), M110-M110 (2011-04-21)
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相关内容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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