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安全信息

P8038

Sigma-Aldrich

细菌蛋白酶

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

别名:

蛋白酶 来源于地衣芽孢杆菌, 枯草肽酶A, 碱性蛋白酶

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About This Item

CAS号:
9014-01-1
EC 号:
3.4.21.62 (BRENDA, IUBMB)
EC 号:
232-752-2
MDL编号:
MFCD00132092
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

类型

Type XXIV

质量水平

300

表单

lyophilized powder

比活

≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid

分子量

27 kDa

纯化方式

crystallization

运输

wet ice

储存温度

−20°C

相关类别

特异性

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白，并在碱性条件下具有活性。

应用

来自sigma的该酶已用于优化大鼠心脏均质化心室组织中所有线粒体群的释放。它还用于福尔马林固定石蜡包埋肝脏样本的预杂交处理，用于人和病毒DNA的检测。
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。 产品P8038，也被称为枯草杆菌蛋白酶Carlsberg，已用于水解心肌细胞以研究心脏线粒体NHE1的沉默。

生化/生理作用

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。

物理属性

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

单位定义

在pH 7.5，37 °C 条件下，一单位的酶每分钟将会水解酪蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。

分析说明

氨基酸分析和等电聚焦电泳与枯草杆菌蛋白酶Carlsberg一致。

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 2

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000413589
0000413589
0000369770
0000375419
0000375420

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the pH optimum for Product P8038, Proteinase, bacterial?

    Two references cite pH 7.5 as optimal for this enzyme: (a) Ricks et al., Biotechnol. Prog., 8, 197-203 (1992), (b) Doswald et al., Bioorg. Med. Chem.,  2, 403-410 (1994).

  6. How long can I store stock solutions of Product P8038, Proteinase, bacterial?

    Our notes indicate that a 0.1% solution (1 mg/mL) in PBS or TBS can be stored for about a week at 0-4°C.  For longer term storage, we would advise freezing the stock solution in aliquots.

  7. What is the pI of Product P8038, Proteinase, bacterial?

    One reference gives a pI of 9.4 for subtilisin Carlsberg. See: Zacharis, E. et al., Proc. Nat. Acad. Sci. USA, 96, 1201-1205 (1999).  

  8. What is the molecular weight (MW) of Product P8038, Proteinase, bacterial?

    The enzyme has a MW of 27.5-27.6 kDa. See DeLange & Smith, J. Biol. Chem., 243(9), 2134-2142 (1968).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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