推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
PSTAIR, monoclonal
分子量
antigen 33-34 kDa
包含
15 mM sodium azide
种属反应性
mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates
技术
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:4,000 using a COS-7 cell extract
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CDC2(983)
mouse ... Cdc2a(12534)
一般描述
在检测的每个真核细胞中，CDK均含有16个氨基酸组成的进化保守序列PSTAIR(EGVPSTAIREISLLKE)，是区别其他蛋白激酶的标志。 PSTAIR基序与周期蛋白共同参与复合物形成。 特异性针对PSTAIR序列的单克隆抗体适用多种生物，可对各种CDK进行亚细胞水平的检测和定位，并可用于研究CDK底物相互作用。
抗-PSTAIR（小鼠IgG1亚型）单克隆抗体来源于小鼠骨髓瘤细胞和BALB/c小鼠脾细胞相互融合而产生的PSTAIR杂交瘤。细胞周期蛋白依赖性激酶1(CDC2/CDK1)属于Ser/Thr蛋白激酶家族。 位于人类染色体10q21。
免疫原
16氨基酸合成寡肽含有BSA标记的PSTAIR序列
应用
小鼠抗-PSTAIR单克隆抗体可用于：
- 蛋白质印迹分析(western blot)
- 酶联免疫吸附测定(ELISA)
- 免疫沉淀
小鼠抗-PSTAIR单克隆抗体（PSTAIR克隆）可用于通过ELISA、免疫印迹和免疫沉淀等各种免疫化学技术，对含PSTAIR基序的周期蛋白依赖性激酶进行定位。
生化/生理作用
周期蛋白依赖性激酶1(CDC2/CDK1)调控着有丝分裂过程的多个环节，包括细胞骨架重组、染色体分离、子细胞的形成和分离。对于哺乳动物细胞的增殖至关重要。参与肿瘤发生。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Martin Převorovský et al.
Cell cycle (Georgetown, Tex.), 15(22), 3082-3093 (2016-10-22)
For every eukaryotic cell to grow and divide, intricately coordinated action of numerous proteins is required to ensure proper cell-cycle progression. The fission yeast Schizosaccharomyces pombe has been instrumental in elucidating the fundamental principles of cell-cycle control. Mutations in S.
Martin Převorovský et al.
PloS one, 10(9), e0137820-e0137820 (2015-09-15)
Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped. Using a combination of transcriptome profiling
Yuko Takayama et al.
Scientific reports, 6, 38111-38111 (2016-12-03)
Histone gene expression is regulated in a cell cycle-dependent manner, with a peak at S phase, which is crucial for cell division and genome integrity. However, the detailed mechanisms by which expression of histone genes are tightly regulated remain largely
Héloïse Chassé et al.
PloS one, 11(3), e0150318-e0150318 (2016-03-11)
The cyclin B/CDK1 complex is a key regulator of mitotic entry. Using PP242, a specific ATP-competitive inhibitor of mTOR kinase, we provide evidence that the mTOR signalling pathway controls cyclin B mRNA translation following fertilization in Sphaerechinus granularis and Paracentrotus
Marie Knockaert et al.
Oncogene, 21(42), 6413-6424 (2002-09-13)
Chemical inhibitors of cyclin-dependent kinases (CDKs) have a great therapeutic potential against various proliferative and neurodegenerative disorders. Intensive screening of a combinatorial chemistry library of 2,6,9-trisubstituted purines has led to the identification of purvalanol, one of the most potent and
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