P7937
蛋白磷酸酶-1 催化亚单位，α-亚型 来源于兔
5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder
别名:
蛋白磷酸酶
产品名称
蛋白磷酸酶-1 催化亚单位，α-亚型 来源于兔, 5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder
technique(s)
inhibition assay: suitable
biological source
rabbit
recombinant
expressed in E. coli
assay
≥80% (GE)
form
lyophilized powder
specific activity
5,000-15,000 units/mg protein
mol wt
37.5 kDa
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
rabbit ... PPP1CA(100009298)
Application
来自家兔的蛋白磷酸酶1催化亚基α同种型已被用作蛋白磷酸酶1抑制试验的标准品。
蛋白磷酸酶-1 催化亚单位，α-家兔的同种型已用于蛋白磷酸酶反应和脂肪细胞提取物的消化。
Biochem/physiol Actions
蛋白磷酸酶 1 (PP1) 参与糖原代谢和肌肉收缩调节。此外，它还参与细胞周期和转录调控。重组兔 PP1α性质与天然兔肌肉蛋白相同，包括大小、活性对 Mn 2 + 的要求、磷酸化酶 A 的特异性以及冈田酸、微囊藻毒素 LR 和磷酸酶抑制剂 2 (I-2) 的抑制作用。
General description
蛋白磷酸酶 1 (PP1) 是一种具有丝氨酸/苏氨酸磷酸酶活性的异二聚体酶。它包括一个催化亚基和一个靶向亚基或一个特异性蛋白抑制剂。靶向亚单位决定酶的定位，例如糖原颗粒（亚单位 g）或肌原纤维（亚单位 M）。
Other Notes
在 pH 7.4 和 30℃ 条件下，1 个单元每分钟水解 1 nmol 对硝基苯基磷酸盐。
Packaging
包装尺寸基于蛋白质含量。
Physical form
冻干粉，含咪唑缓冲液 (pH 7.4)、NaCl、DTT、EDTA、MnCl 2 、吐温 20 和海藻糖作为稳定剂。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Regulation of protein phosphatase-1
James B Aggen
Chemistry and Biology, 7.1, R13-R23 (2000)
Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.
T Hunter
Cell, 80(2), 225-236 (1995-01-27)
Activity of membrane-associated sucrose synthase is regulated
by its phosphorylation status in cultured cells of sycamore (Acer
pseudoplatanus)
by its phosphorylation status in cultured cells of sycamore (Acer
pseudoplatanus)
Javier P
Physiologia Plantarum (2004)
Satomi Ohtsuka et al.
Biochemistry, 59(17), 1701-1710 (2020-04-17)
Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) activates particular multifunctional kinases, including CaMKI, CaMKIV, and 5'AMP-activated protein kinase (AMPK), resulting in the regulation of various Ca2+-dependent cellular processes, including neuronal, metabolic, and pathophysiological pathways. We developed and characterized a novel pan-CaMKK inhibitor
A J Zhang et al.
The Journal of biological chemistry, 267(3), 1484-1490 (1992-01-25)
The catalytic subunit of rabbit skeletal muscle protein phosphatase-1 was expressed in Escherichia coli. Expression of phosphatase-1 in the pET3a vector, which is based on the use of the T7 promoter, resulted in the expression of the enzyme as an
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