产品名称
Anti-Peroxidase antibody produced in rabbit, fractionated antiserum, lyophilized powder
biological source
rabbit
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
horseradish
technique(s)
indirect ELISA: 1:100,000-1:200,000
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Application
Anti-Peroxidase antibody produced in rabbit has been used in:
- immunoblot assays
- enzyme-linked-immunosorbent assay (ELISA)
- immunoprecipitation
Biochem/physiol Actions
Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Immunogen
Purified peroxidase from horseradish
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Preparation Note
Reconstitute with deionized water.
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress
Jain P and Bhatla SC
PLoS ONE, 13(5), e0197132-e0197132 (2018)
Bin Yang et al.
Oncology letters, 15(4), 4662-4668 (2018-03-16)
The present study aimed to assess whether different anesthesia methods (general anesthesia and general anesthesia combined with epidural block) were associated with tumor metastasis during the perioperative period and the possible molecular mechanisms of tumor metastasis. A rat hepatoma tumor
Chilling injury and peroxidase activity changes in ?Fortune? mandarin fruit during low temperature storage
El-HF, et al.
Bulgarian Journal of Plant Physiology, 29(1-2), 44-54 (2003)
Koto Furotani et al.
PloS one, 13(9), e0204048-e0204048 (2018-09-19)
APP (amyloid precursor protein), the causative molecule of Alzheimer's disease, is synthesized in neuronal cell bodies and subsequently transported to synapses. We previously showed that the yata gene is required for the synaptic transport of the APP orthologue in Drosophila
Mary Lynn Dear et al.
Development (Cambridge, England), 143(1), 75-87 (2015-11-26)
Synaptogenesis requires orchestrated intercellular communication between synaptic partners, with trans-synaptic signals necessarily traversing the extracellular synaptomatrix separating presynaptic and postsynaptic cells. Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt
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