P7173
Pyruvate Carboxylase from bovine liver
buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)
别名:
Pyruvate:CO2 ligase (ADP-forming)
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10 G
¥1,130.45
50 G
¥4,438.31
100 G
¥6,412.96
500 G
¥22,101.03
¥1,130.45
国内现货，预计发货时间2025年4月24日详情
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10 G
¥1,130.45
50 G
¥4,438.31
100 G
¥6,412.96
500 G
¥22,101.03
About This Item
¥1,130.45
国内现货，预计发货时间2025年4月24日详情
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此商品 | D4626 | D5376 | D8821 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility water: 0.1 g/mL, colorless to faintly yellow | solubility water: 20 mg/mL, clear, colorless to faintly yellow | solubility - | solubility water: 100 mg/mL at 25 °C, colorless to faintly yellow |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| optical activity [α]/D 199° | optical activity [α]/D 199° | optical activity [α]/D 199° | optical activity [α]20/D 190 to 201 °, c = 2% (w/v) in water |
应用
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.[1]
生化/生理作用
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA[2].
单位定义
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.
外形
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
制备说明
亲和纯化
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Philip Lee et al.
Hepatology (Baltimore, Md.), 57(2), 515-524 (2012-08-23)
The pathogenesis of type 2 diabetes is characterized by impaired insulin action and increased hepatic glucose production (HGP). Despite the importance of hepatic metabolic aberrations in diabetes development, there is currently no molecular probe that allows measurement of hepatic gluconeogenic
Eunsook S Jin et al.
Metabolism: clinical and experimental, 62(1), 152-162 (2012-09-18)
A three-day high-fat diet induces hepatic steatosis and hepatic insulin resistance in rats without altering fasting plasma glucose concentration or the rate of glucose production. However, as the nutrient profile available to the liver is substantially altered by a high-fat
M L Lazo de la Vega-Monroy et al.
The Journal of nutritional biochemistry, 24(1), 169-177 (2012-07-31)
Besides its role as a carboxylase cofactor, biotin has a wide repertoire of effects on gene expression, development and metabolism. Pharmacological concentrations of biotin enhance insulin secretion and the expression of genes and signaling pathways that favor islet function in
H M White et al.
Journal of dairy science, 95(3), 1249-1256 (2012-03-01)
Expression of mRNA for pyruvate carboxylase (PC) is elevated at calving and during other physiological states when plasma NEFA concentrations are increased. The objective of this study was to determine the direct effects of fatty acids on expression of PC
Abdussalam Adina-Zada et al.
Biochemistry, 51(41), 8208-8217 (2012-09-19)
Mutation of Arg427 and Arg472 in Rhizobium etli pyruvate carboxylase to serine or lysine greatly increased the activation constant (K(a)) of acetyl CoA, with the increase being greater for the Arg472 mutants. These results indicate that while both these residues
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