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Merck
CN

P7119

Sigma-Aldrich

PGO酶制剂

1 G capsules

别名:

Peroxidase - Glucose oxidase preparation

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About This Item

UNSPSC代码:
12352204
NACRES:
NA.54

质量水平

形式

powder

溶解性

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

储存温度

2-8°C

一般描述

每个胶囊含有500单位的葡萄糖氧化酶(Aspergilus niger)、100个purpurogallin单位的过氧化物酶(辣根)和缓冲盐。该反应产生棕色的氧化邻联茴香胺。在425-475nm处测量的棕色强度与原始葡萄糖浓度成比例。

应用

PGO酶已被用于:
  • 饮食抽样和化学分析
  • 测定血浆和卵泡液的葡萄糖水平
  • 测定脱支回生米淀粉组分中的葡萄糖含量

生化/生理作用

PGO酶用于酶法定量测定水溶液(如血清)中的葡萄糖。通常用邻联茴香胺作为比色底物,在425-475nm处监测反应。葡萄糖氧化酶法消除了干扰物质的影响。一个胶囊的内容物包含100单位过氧化物酶和500单位葡萄糖氧化酶以及缓冲盐。

适用性

经测试适用于葡萄糖的定量。

制备说明

将1个胶囊PGO酶的内容物加入到琥珀色瓶中的100mL水中,制备PGO酶溶液。将瓶子颠倒几次,轻轻摇动溶解。PGO酶溶液应储存在2-8°C。除非产生浑浊,否则溶液可稳定保存长达1个月,在-20°C下至少可保存6个月。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

含少量动物源组分生物产品
常规特殊物品

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