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安全信息

P5860

Sigma-Aldrich

蛋白酶 来源于芽孢杆菌

liquid, ≥8 U/g

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别名:
Esperase® 8.0L
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

形式

liquid

质量水平

比活

≥8 U/g

分子量

20--30 kDa

储存温度

2-8°C

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相关类别

应用

蛋白酶是一种通过水解肽键而分解蛋白质的酶。产物P5860是从芽孢杆菌属(Bacillus sp.)中分泌的。它是一种丝氨酸型蛋白酶,在高温和高pH下具有出色的活性。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热灭活动力学。 该酶已被作为一种环境友好的非热技术,用于测试紫外线辐射,以便对食品工业中的酶进行灭活。

生化/生理作用

蛋白酶通过水解肽键而分解蛋白质。丝氨酸活性位点抑制剂(如苯基甲基磺酰氟(PMSF)和氟磷酸二异丙酯)可使蛋白酶失活。从芽孢杆菌属(Bacillus sp.)分泌的蛋白酶的分子量通常为20,000至30,000。它们通常由Ca2+ 稳定,并具有较高的等电点

法律信息

Novozyme 公司产品。
Esperase is a registered trademark of Novozymes Corp.

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

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Materials science & engineering. C, Materials for biological applications, 82, 210-216 (2017-10-14)
Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL
D R Durham et al.
Journal of bacteriology, 169(6), 2762-2768 (1987-06-01)
An alkalophilic Bacillus sp., strain GX6638 (ATCC 53278), was isolated from soil and shown to produce a minimum of three alkaline proteases. The proteases were purified by ion-exchange chromatography and were distinguishable by their isoelectric point, molecular weight, and electrophoretic
Effect of UV light on microbial proteases: From enzyme inactivation to antioxidant mitigation.
Lante A, et al.
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Ultrasonics sonochemistry, 68, 105201-105201 (2020-06-11)
Protein is one of the most abundant natural polymeric materials, but only a few studies on nanoproteins have been conducted. In this paper, a novel approach based on ultrasound-assisted enzymatic hydrolysis was employed for the preparation of microkeratin and nanokeratin
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International journal of biological macromolecules, 85, 476-486 (2016-01-13)
The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by (31)P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation

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