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Merck
CN

P5744

Sigma-Aldrich

磷酸酶底物

powder

别名:

4-硝基苯磷酸盐 二钠盐 六水合物, pNPP 二钠盐 六水合物

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About This Item

线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
分子量:
371.14
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

产品名称

磷酸酶底物, 40 mg capsules

质量水平

表单

powder

储存温度

−20°C

SMILES字符串

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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应用

用于碱性和酸性磷酸酶ELISA法的发色底物。产生可溶性终产物,黄色,分光光度法在405nm处读取。pNPP反应能被3N NAOH终止,在405nm处读取。

其他说明

酸性和碱性磷酸酶实验底物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ahmed G Abu-Shahba et al.
Tissue engineering and regenerative medicine, 17(4), 477-493 (2020-05-26)
Insufficient vascularization hampers bone tissue engineering strategies for reconstructing large bone defects. Delivery of prolyl-hydroxylase inhibitors (PHIs) is an interesting approach to upregulate vascular endothelial growth factor (VEGF) by mimicking hypoxic stabilization of hypoxia-inducible factor-1alpha (HIF-1α). This study assessed two
Elizabeth A Shirtcliff et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(8), 2963-2967 (2009-02-04)
It is well known that children need solicitous parenting and a nurturing rearing environment to ensure their normal behavioral development. Early adversity often negatively impacts emotional and mental well-being, but it is less clearly established how much the maturation and
Rasmus Iversen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17146-17151 (2014-11-19)
The multifunctional enzyme transglutaminase 2 (TG2) is the target of autoantibodies in the gluten-sensitive enteropathy celiac disease. In addition, the enzyme is responsible for deamidation of gluten peptides, which are subsequently targeted by T cells. To understand the regulation of
Masaya Yokota et al.
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not
Adeline Guais et al.
Gene, 374, 112-120 (2006-03-22)
In Drosophila, the RING finger protein d-Goliath was originally identified as a transcription factor involved in the embryo mesoderm formation [Bouchard, M.L., Cote, S., 1993. The Drosophila melanogaster developmental gene g1 encodes a variant zinc-finger-motif protein. Gene 125, 205-209]. In

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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