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Merck
CN

P5459

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

glycerol solution (50%)

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别名:
Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌
CAS号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

Bacillus licheniformis

质量水平

形式

glycerol solution (50%)

比活

≥7 units/mg protein (biuret)

浓度

≥50 mg/mL

溶解性

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

储存温度

2-8°C

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相关类别

一般描述

该酶在50℃下、pH 8-10的范围内具有最佳的明胶水解效果。商业上,它在50℃下、pH 6-10之间是稳定的,被用于用于洗衣洗涤剂配方,也用于从照相乳剂中回收银。

应用

使用来自地衣芽孢杆菌的蛋白酶来消化蛾触角叶中的神经外膜;绿棉铃虫
蛋白酶是一种通过水解肽键来分解蛋白的酶。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。来自地衣芽孢杆菌的蛋白酶(商品货号P5459)用于从骨胳肌中分离线粒体时水解肌肉。

生化/生理作用

蛋白酶通过肽键的水解使蛋白质分解代谢。蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

包装

包装规格基于蛋白含量。

单位定义

若无其他说明,则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色(使用Folin-Ciocalteu试剂显色)所需的酶量。

象形图

Health hazardCorrosion

警示用语:

Danger

危险声明

危险分类

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

WGK

WGK 2

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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