推荐产品
表单
lyophilized powder
比活
≥150 units/mg protein
组成
Protein, 20-50% biuret
环保替代产品特性
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
环保替代产品分类
, Enabling
储存温度
2-8°C
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此商品 | B9285 | D5412 | I7125 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥99% (HPLC) | assay ≥99% | assay ≥98.5% | assay ≥99% (HPLC) |
| form powder | form powder | form powder | form powder or crystals |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. 2-8°C |
| solubility DMSO: 50 mg/mL, clear, colorless to very faintly yellow | solubility NH4OH: 0.1 M at 20 °C, clear, colorless | solubility water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility 1 N NH4OH: 50 mg/mL, clear to very slightly hazy, colorless to light yellow |
一般描述
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品已用作替代能源的酶。更多信息，请参阅 替代能源的酶研究
应用
在测定沙棘果汁中果胶甲酯酶活性时，使用 Sigma 酶作为标准。[1]它被用于细胞壁果胶的水解，同时研究 拟南芥中的叶状结构 。[2]它也被用于催化果胶水解（使用超声波测量装置），以监测生物过程。[3]
果胶酯酶用于将果胶的甲酯水解为果胶酯和甲醇。含有果胶甲酯酶和果胶果胶水解酶。产品 P5400 来自橘皮，为冻干粉。包含 (NH4)2SO4 和氯化钠。已用于果胶甲基酯酶基因的研究 [4]。
生化/生理作用
果胶酯酶催化果胶的甲酯水解产生果胶和甲醇。
单位定义
在 pH 7.5、30°C 条件下，1 个单位每分钟从果胶中释放 1.0 μg 酸。
外形
含 (NH4)2SO4 和氯化钠
分析说明
由缩二脲测定的蛋白质。
其他说明
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
Ida Barbara Reca et al.
Plant molecular biology, 79(4-5), 429-442 (2012-05-23)
A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects
Mélanie L'Enfant et al.
International journal of biological macromolecules, 132, 1140-1146 (2019-04-13)
The discovery of molecules that can inhibit the action of phytopathogens is essential to find alternative to current pesticides. Pectin methylesterases (PME), enzymes that fine-tune the degree of methylesterification of plant cell wall pectins, play a key role in the
Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
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