P5400
果胶酯酶 来源于橘皮
lyophilized powder, ≥150 units/mg protein
别名:
Pectin methylesterase, 果胶甲酯酶
产品名称
果胶酯酶 来源于橘皮, lyophilized powder, ≥150 units/mg protein
form
lyophilized powder
specific activity
≥150 units/mg protein
composition
Protein, 20-50% biuret
greener alternative product characteristics
Design for Energy Efficiency
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sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
Quality Level
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相关类别
Other Notes
在 pH 7.5、30°C 条件下，1 个单位每分钟从果胶中释放 1.0 μg 酸。
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
Analysis Note
由缩二脲测定的蛋白质。
Application
在测定沙棘果汁中果胶甲酯酶活性时，使用 Sigma 酶作为标准。它被用于细胞壁果胶的水解，同时研究 拟南芥中的叶状结构 。它也被用于催化果胶水解（使用超声波测量装置），以监测生物过程。
果胶酯酶用于将果胶的甲酯水解为果胶酯和甲醇。含有果胶甲酯酶和果胶果胶水解酶。产品 P5400 来自橘皮，为冻干粉。包含 (NH4)2SO4 和氯化钠。已用于果胶甲基酯酶基因的研究 。
Biochem/physiol Actions
果胶酯酶催化果胶的甲酯水解产生果胶和甲醇。
General description
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品已用作替代能源的酶。更多信息，请参阅 替代能源的酶研究
Physical form
含 (NH4)2SO4 和氯化钠
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
Sebastian Wolf et al.
Current biology : CB, 22(18), 1732-1737 (2012-08-14)
Brassinosteroid (BR) signaling is required for normal plant growth as shown by the dwarf phenotype of loss-of-function BR biosynthetic or perception mutants. Despite a detailed understanding of the BR signaling network, it is not clear how exactly BRs control growth.
On-line characterization using ultrasound of pectin hydrolysis catalyzed by the enzyme pectinmethylesterase.
Cristina A, et al.
IOP Conference Series: Materials Science and Engineering, 42 (2012)
Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
Mélanie L'Enfant et al.
International journal of biological macromolecules, 132, 1140-1146 (2019-04-13)
The discovery of molecules that can inhibit the action of phytopathogens is essential to find alternative to current pesticides. Pectin methylesterases (PME), enzymes that fine-tune the degree of methylesterification of plant cell wall pectins, play a key role in the
Daniele Del Corpo et al.
Molecular plant pathology, 21(12), 1620-1633 (2020-10-09)
Pectin is synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by pectin methylesterases (PMEs). Arabidopsis thaliana produces a local and strong induction of PME activity during the infection of the necrotrophic fungus Botrytis
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