应用
在 体外 条件下,Sigma的该酶被用于鞘氨酸激酶1 (SK1)向膜组分的转移。它还被用于从HL60透化性细胞中的磷脂酰胆碱(PC)产生磷脂酸(PA)。
脂酶D(PLD)已被用作水解磷脂与鞘磷脂的磷酸键,得到相应的磷脂酸。 它已可用于研究代谢标记和 体内 直接成像胆碱磷脂,通过测量propargyl-cho的掺入。此外,PLD已可用于纯化和动力学研究。
生化/生理作用
磷脂酶D是甘油磷脂特异性的。 它对鞘磷脂和溶血磷脂的活性明显降低。 磷脂酶D水解磷脂和鞘磷脂的磷酸键,从而得到相应的磷脂酸。
水解磷脂和鞘磷脂的磷酸键,获得相应的磷脂酸。
包装
无底玻璃瓶。内容物在插入的熔锥内。
单位定义
在pH为 8.0,温度是37 °C的条件下,一个单位酶每小时可从1,2-二油基-{1}sn{2}-甘油-3-磷酸胆碱释放1.0 μmole的胆碱。
分析说明
蛋白质由双缩脲法测定
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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