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类型
Type XXVII
表单
lyophilized powder
比活
7-14 units/mg solid
纯化方式
crystallization
异质活性
DNase, RNase, essentially free
储存温度
−20°C
应用
Protease from Bacillus sp. has been used for enzymatic antigen retrieval during immunohistochemical studies. It has also been used in protease treatment for the isolation of mitochondria from muscle specimens.
生化/生理作用
Protease is widely used in various industries such as the leather industry, food industry, and pharmaceutical industry. It is used in laundry detergents to remove protein-based stains from clothes. In addition, protease also helps in the wound healing process by removing necrotic materials. It also finds application in contact-lens enzyme cleaners and enzymatic deriders. Nargase, a protease isolated from Bacillus sp. is widely used for isolation of interfibrillar (IFM) mitochondria.
单位定义
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
分析说明
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin BPN′.
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
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Nagarse treatment of cardiac subsarcolemmal and interfibrillar mitochondria accounts for inaccurate quantification of proteins
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Journal of Molecular and Cellular Cardiology, 120, 5-6 (2018)
L H Lash et al.
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Selective permeabilization of plasma membranes with digitonin produced separation of cytosolic and mitochondrial compartments of proximal tubular (PT) and distal tubular (DT) cells from a rat kidney. Subcellular distributions of several intracellular glutathione (GSH)-dependent enzymes were similar in the two
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