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The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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P4664

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

别名:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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1 ML
¥1,076.08
5 ML
¥4,941.99

¥1,076.08

目录价¥1,506.50节省 29%

预计发货时间2025年4月27日详情

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变更视图
1 ML
¥1,076.08
5 ML
¥4,941.99

About This Item

线性分子式:
C6H4(NH2)2·2HCl
CAS号:
615-28-1
分子量:
181.06
Beilstein:
3912045
MDL编号:
MFCD00012966
UNSPSC代码:
12352204
PubChem化学物质编号:
24898491
NACRES:
NA.83

¥1,076.08

目录价¥1,506.50节省 29%

预计发货时间2025年4月27日详情

产品名称

邻苯二胺 二盐酸盐, tablet, 15 mg substrate per tablet

质量水平

200

表单

tablet

mp

258 °C (dec.) (lit.)

溶解性

water: 1 tablet/10 mL, clear, colorless

储存温度

2-8°C

SMILES字符串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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form

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

form

DMSO solution

form

lyophilized powder

form

lyophilized

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

100

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

一般描述

邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。

应用

邻苯二胺盐酸盐已用于：
  • 作为传统捕获底物，用于重组血凝素（rHA）蛋白的酶联免疫吸附法检测（ELISA）
  • 作为底物，通过ELISA法测定严重急性呼吸综合征冠状病毒2（SARS-CoV-2）免疫球蛋白G（IgG）抗体
  • 作为特异性比色试剂，利用S蛋白的SARS-CoV-2抗原在ELISA方案中进行辣根过氧化物酶（HRP）活性评估

重悬

将 1 片片剂溶于 0.05 M 磷酸盐-柠檬酸盐缓冲液 (pH 5.0) 中，得到所需浓度（通常 OPD 浓度为 使用 0.4 mg/mL）。在使用前即刻给每 100ml 底物缓冲溶液加入 40μl 新鲜的 30% 过氧化氢。片剂分别装在箔包中。

警示用语：

Warning

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000390834
    0000381937
    0000381936
    0000310684
    0000310689

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    Ying-Feng Chang et al.
    Biosensors & bioelectronics, 26(3), 1068-1073 (2010-09-22)
    Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed
    B Abomoelak et al.
    Infection and immunity, 67(10), 5100-5105 (1999-09-25)
    The development of combined vaccines constitutes one of the priorities in modern vaccine research. One of the most successful combined vaccines in use is the diphtheria-pertussis-tetanus vaccine. However, concerns about the safety of the pertussis arm have led to decreased
    Hepatitis B virus transgenic severe combined immunodeficient mouse model of acute and chronic liver disease.
    Mark A Feitelson et al.
    Methods in molecular medicine, 96, 269-287 (2004-02-06)
    E Werle et al.
    The Journal of rheumatology, 28(3), 595-600 (2001-04-12)
    To determine the prevalence and potential diagnostic relevance of autoantibodies against serotonin, thromboplastin, and ganglioside Gm1 in patients with fibromyalgia syndrome (FM). Sera from 203 patients with FM and 64 pain-free control subjects were analyzed with enzyme immunoassays. Clinical and
    Greg Davis et al.
    PloS one, 16(7), e0254667-e0254667 (2021-07-15)
    The world is currently in a pandemic of COVID-19 (Coronavirus disease-2019) caused by a novel positive-sense, single-stranded RNA β-coronavirus referred to as SARS-CoV-2. Here we investigated rates of SARS-CoV-2 infection in the greater Cincinnati, Ohio, USA metropolitan area from August
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