P4105
丙酮酸氧化酶 来源于气球菌 属
lyophilized powder, ≥35 units/mg protein (biuret)
别名:
丙酮酸：氧氧化还原酶（磷酸化）
产品名称
丙酮酸氧化酶 来源于气球菌 属, lyophilized powder, ≥35 units/mg protein (biuret)
biological source
bacterial (Aerococcus spp.)
form
lyophilized powder
specific activity
≥35 units/mg protein (biuret)
storage temp.
−20°C
Quality Level
Application
丙酮酸氧化酶（PoxB）可将丙酮酸直接转化为乙酸盐和CO2。它可用于研究丙酮酸的代谢。用于研究细菌的有氧代谢，例如 植物乳杆菌和 肺炎链球菌。
来自气球菌的丙酮酸氧化酶已被用于丙酮酸的基于Amplex Red的荧光分析中。 它适用于制备用于测定硫胺素（维生素B1）的生物传感器， 以及作为多酶生物催化级联反应恒定部分的组成部分，从而用于确定创伤性脑损伤（TBI）和软组织损伤（STI）的生物标志物。
Biochem/physiol Actions
丙酮酸氧化酶可将丙酮酸氧化形成乙酸盐、过氧化氢和二氧化碳。 丙酮酸氧化酶需要黄素腺嘌呤二核苷酸（FAD）、焦磷酸硫胺素（TPP）和镁作为其催化活性的辅助因子。硫胺素可激活丙酮酸氧化酶活性。
丙酮酸氧化酶由四个具有相同分子量的亚基组成。PoxB可与某些醛反应，并且磷酸盐可以被砷酸盐代替。氧气以及几种人造化合物都可以充当电子受体。丙酮酸氧化酶可被磷脂以及单体和胶束两亲物活化。
Other Notes
在37°C、pH 6.7 条件下，在丙酮酸和磷酸盐转化为乙酰磷酸盐和 CO2 的过程中，一单位丙酮酸氧化酶每分钟可产生 1.0 μmole H2O2。
Physical form
含有缓冲盐和糖的冻干粉
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Multi-enzyme logic network architectures for assessing injuries: digital processing of biomarkers
Halamek J, et al.
Molecular Biosystems, 6(12), 2554-2560 (2010)
B Sedewitz et al.
Journal of bacteriology, 160(1), 462-465 (1984-10-01)
Under aerobic growth conditions Lactobacillus plantarum produced acetic acid in addition to lactic acid. It was found that lactic acid was predominantly produced at first, and then when the carbohydrate was nearly exhausted, lactic acid was metabolized further to acetic
Hiroaki Taniai et al.
Journal of bacteriology, 190(10), 3572-3579 (2008-03-18)
Streptococcus pneumoniae was shown to possess lactate oxidase in addition to well-documented pyruvate oxidase. The activities of both H(2)O(2)-forming oxidases in wild-type cultures were detectable even in the early exponential phase of growth and attained the highest levels in the
B Sedewitz et al.
Journal of bacteriology, 160(1), 273-278 (1984-10-01)
Pyruvate oxidase (EC 1.2.3.3) was isolated and characterized from Lactobacillus plantarum. The enzyme catalyzes the oxidative decarboxylation of pyruvate in the presence of phosphate and oxygen, yielding acetyl phosphate, carbon dioxide, and hydrogen peroxide. This pyruvate oxidase is a flavoprotein
Activation of pyruvate oxidase by monomeric and micellar amphiphiles.
R Blake et al.
The Journal of biological chemistry, 253(6), 1963-1971 (1978-03-25)
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