P4069
碱性磷酸酶 来源于大肠杆菌
buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
Specific activity:
20-50 units/mg protein (in glycine buffer)
Biological source:
Escherichia coli
产品名称
碱性磷酸酶 来源于大肠杆菌, buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
biological source
Escherichia coli
form
buffered aqueous glycerol solution
specific activity
20-50 units/mg protein (in glycine buffer)
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Quality Level
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Analysis Note
缩二脲法测定蛋白
Application
来自 大肠杆菌 的碱性磷酸酶已被用于：
- 荧光测定法中测定其在去磷酸化酶混合物中的酶活性
- 用于处理神经和 HL60 细胞裂解物中
- 的 β-羟基 β-甲基戊二酰辅酶 A (HMG-CoA) 还原酶活性
碱性磷酸酶用于偶联抗体和其他蛋白，用于 ELISA、免疫印迹和组织化学检测。需要高灵敏度时可用于蛋白标记。产品 P4069 以甘油缓冲水溶液形式提供。
Biochem/physiol Actions
碱性磷酸酶 (Alkaline phosphatase)，来源于大肠杆菌，是一种主要存在于周质空间的二聚体、非糖基化蛋白质。已知存在三种亚型。这种酶需要锌，并被镁激活。大肠杆菌含皂碱磷酸酶对磷酸酯具有广泛的特异性。
碱性磷酸酶是研究磷酸酯酶的模型酶。用于各种生化方法和酶联免疫吸附试验 (ELISA)。
Disclaimer
5 mM EDTA (pH 8) 中的碱性磷酸酶将在 75℃ 下不可逆地热灭活 10 min。
Other Notes
一个单位将在37°C 、 pH 10.4 下每分钟水解 1.0μ摩尔 对 -硝基苯磷酸盐。
Physical form
5 mM Tris-HCl 溶液，pH 7.4，0.5 mM MgCl 2 ，0.5 mM ZnCl 2 ，50% (w/v) 甘油
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
The prion protein and cellular cholesterol homeostasis
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Neurobiology of Lipids, 1(2), 8-14 (2002)
Sensitive fluorogenic substrate for alkaline phosphatase
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Phospho-iTRAQ data article: Assessing isobaric labels for the large-scale study of phosphopeptide stoichiometry
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Data in Brief, 4(2), 60-65 (2015)
Lionel M Igaz et al.
The American journal of pathology, 173(1), 182-194 (2008-06-07)
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