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Merck
CN

P4032

Sigma-Aldrich

朊酶 来源于蜂蜜曲霉

Type XXIII, ≥3 units/mg solid

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别名:
Protease from Aspergillus melleus, Proteinase from Aspergillus sp.
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

Aspergillus sp. (A. melleus)

质量水平

类型

Type XXIII

形式

solid

比活

≥3 units/mg solid

储存温度

2-8°C

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相关类别

应用

蛋白酶是一种通过水解肽键来分解蛋白质的酶。蛋白酶用于降解蛋白质、研究蛋白酶抑制剂和研究热灭活动力学。蛋白酶用于孵育过程中的核酸分离程序。它被用于研究蛋白酶激活受体,如炎症和免疫反应中蛋白酶介导的信号转导 。产品 P4032 来自 黑曲霉 ,已用于霍氏链霉菌木聚糖酶的非特异性降解。

生化/生理作用

蛋白酶通过水解肽键使蛋白质分解代谢。丝氨酸活性部位抑制剂可灭活蛋白酶,如苯甲基磺酰氟 (PMSF) 和二异丙基氟磷酸盐。

单位定义

除非另有说明,否则一个单位将水解酪蛋白以在 pH7.5,37°C 下每分钟产生相当于1.0μmole (181μg) 酪氨酸的颜色(Folin-Ciocalteu 试剂的颜色)。

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

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Serine proteinases such as thrombin, mast cell tryptase, trypsin, or cathepsin G, for example, are highly active mediators with diverse biological activities. So far, proteinases have been considered to act primarily as degradative enzymes in the extracellular space. However, their
Chenzhong Yin et al.
Scientific reports, 10(1), 15078-15078 (2020-09-17)
Understanding the mechanisms by which neurons create or suppress connections to enable communication in brain-derived neuronal cultures can inform how learning, cognition and creative behavior emerge. While prior studies have shown that neuronal cultures possess self-organizing criticality properties, we further
Ravindra S Prajapati et al.
Development (Cambridge, England), 146(24) (2019-12-07)
During early embryogenesis, the ectoderm is rapidly subdivided into neural, neural crest and sensory progenitors. How the onset of lineage determinants and the loss of pluripotency markers are temporally and spatially coordinated in vivo is still debated. Here, we identify
Mikhail E Kandel et al.
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Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field and absorption attenuates light that travels long optical paths. These obstacles limit
Laura E Mickelsen et al.
eLife, 9 (2020-10-30)
The ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance, and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH

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