生物来源
rabbit
质量水平
检测方案
≥95% (SDS-PAGE)
形式
solution
分子量
700 kDa
UniProt登记号
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
rabbit ... PSMA1(100352563) , PSMB1(100343622)
一般描述
Manufactured for Sigma by Boston Biochem., Inc.
应用
20S proteasome fraction from rabbit has been used as a positive control to determine proteasome activity.
生化/生理作用
Catalytic core of the 26S proteasome that degrades polyubiquitinated proteins.
Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP dependent process.
外形
Solution in 50 mM HEPES, pH 7.6, 150 mM sodium chloride, and 1 mM DTT, pH 7.6.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
R Hough et al.
The Journal of biological chemistry, 262(17), 8303-8313 (1987-06-15)
We have purified two high molecular weight proteases approximately 400-fold from rabbit reticulocyte lysate. Both enzymes hydrolyze 125I-alpha-casein and 4-methylcoumaryl-7-amide peptides with tyrosine, phenylalanine, or arginine at the P1 position. Both are inhibited by hemin, thiol reagents, chymostatin, and leupeptin.
R L Stein et al.
Biochemistry, 35(13), 3899-3908 (1996-04-02)
In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized
Examination of `lipotoxicity? in skeletal muscle of high-fat fed and ob/ob mice
S.M. Turpin
The Journal of Physiology (2009)
S M Turpin et al.
The Journal of physiology, 587(Pt 7), 1593-1605 (2009-02-11)
Excess lipid accumulation resulting from an elevated supply of plasma fatty acids is linked to the pathogenesis of the metabolic syndrome and heart disease. The term 'lipotoxicity' was coined to describe how lipid accumulation leads to cellular dysfunction and death
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