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Merck
CN

P3910

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

别名:

Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.84

表单

lyophilized powder

质量水平

质量

for use in Total Dietary Fiber Assay, TDF-100A

分子量

27 kDa

应用

food and beverages

运输

wet ice

储存温度

2-8°C

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特异性

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白,并在碱性条件下具有活性。

应用

这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶,并在碱性条件下具有活性。它用于总膳食纤维检测(TDF-100A)中。

生化/生理作用

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

物理属性

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

单位定义

在pH 7.5、37°C条件下,一个单位每分钟可水解酪蛋白,生成相当于1.0 μmole (181 μg)酪氨酸的颜色。

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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分析证书(COA)

Lot/Batch Number

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