生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
fractionated antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
lyophilized powder
包装
vial of 2 mL lyophilized antiserum
技术
indirect ELISA: 1:100,000
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
分级过程主要得到抗血清的免疫球蛋白部分。为了确保特异性,可根据需要使用固相技术吸附分级的抗血清。兔抗蛋白A抗体是从pH 7.2的0.01M磷酸盐缓冲盐水中冻干的,未添加防腐剂。通过免疫电泳,确定抗体的同一性和纯度。抗体制剂和抗兔IgG经过电泳和随后的扩散,抗体制剂产生单一的沉淀弧,而抗兔IgG全血清产生多个沉淀弧。
蛋白质A是一种42 kDa的细菌蛋白。它是从金黄色酿脓葡萄球菌的细胞壁中提取获得。
免疫原
来自金黄色葡萄球菌的蛋白A
应用
兔抗蛋白质A抗体已用于免疫印迹。
蛋白A可用于免疫印迹,最小稀释度为1:50,000。
生化/生理作用
Protein A 能够与来自多个物种的免疫球蛋白Fc区结合。它可用作“通用示踪剂”,有助于结合配体和自由配体的分离。此外,Protein A也可作为抗体(IgG)纯化的亲和基质。
外形
从pH7.2的0.01M磷酸盐缓冲盐水中冻干
重悬
用 2ml 去离子水复原。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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PloS one, 7(10), e46864-e46864 (2012-10-12)
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Radioimmunoassay
Immunoassay, 269-285 (1996)
Autophagy, 10(9), 1565-1578 (2014-07-22)
Autophagy is an intracellular degradation process that delivers cytosolic material to lysosomes and vacuoles. To investigate the mechanisms that regulate autophagy, we performed a genome-wide screen using a yeast deletion-mutant collection, and found that Npr2 and Npr3 mutants were defective
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