P3752
酸性磷酸酶 来源于马铃薯
lyophilized powder, ≥0.5 unit/mg solid
别名:
(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Specific activity:
≥0.5 unit/mg solid
Biological source:
potato
biological source
potato
Quality Level
form
lyophilized powder
specific activity
≥0.5 unit/mg solid
mol wt
69 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
Application
酸性磷酸酶通常用于蛋白质和核酸的去磷酸化。 酸性磷酸酶是一种酸性水解酶，通常存在于溶酶体中。它可作为鉴定亚细胞组分中溶酶体的标记物。 酸性磷酸酶（产品P3752）已被作为对照，用于研究 牛分枝杆菌 BCG如何影响肺泡巨噬细胞中酸性磷酸酶的活性水平。
Biochem/physiol Actions
酸性磷酸酶是一种细胞质溶酶体水解酶，可催化正磷酸单酯和水转化为醇和正磷酸盐。
Other Notes
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
Filipe Natalio et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 23(21), 4973-4980 (2017-03-02)
Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid
I N Mbawuike et al.
Infection and immunity, 51(2), 483-489 (1986-02-01)
The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10
K Zyła et al.
The British journal of nutrition, 74(1), 3-17 (1995-07-01)
An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3752-1G | 04061835506552 |
| P3752-100MG | 04061834372837 |