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安全信息

P3738

Sigma-Aldrich

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

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别名:
cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
EC 号:
2.7.11.12 (BRENDA, IUBMB)
MDL编号:
MFCD03458230
UNSPSC代码:
12352204
NACRES:
NA.54

重组

expressed in baculovirus infected Sf9 cells

质量水平

200

检测方案

≥95% (SDS-PAGE)

形式

buffered aqueous glycerol solution

比活

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

分子量

76 kDa (monomer)

UniProt登记号

Q13976

相关疾病

cancer

运输

wet ice

储存温度

−20°C

基因信息

human ... PRKG1(5592)

应用

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

生化/生理作用

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

单位定义

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

外形

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Atsuko Deguchi et al.
Cancer research, 65(18), 8442-8447 (2005-09-17)
Recent studies indicate that the induction of apoptosis in human colon cancer cells by certain nonsteroidal antiinflammatory drugs involves increased expression of 15-LOX-1 and synthesis of its major product 13-S-hydroxyoctadecadienoic acid (13-S-HODE). Evidence was obtained that this occurs via a
Faranak Fallahian et al.
Cell biochemistry and function, 30(3), 183-190 (2011-11-19)
Activation of protein kinase G (PKG) by cyclic guanosine 3,5-monophosphate (cGMP) has become of considerable interest as a novel molecular approach for the induction of apoptosis in cancer cells. This study was conducted to investigate the role of PKG isoforms
D Pöhler et al.
FEBS letters, 374(3), 419-425 (1995-11-06)
Detailed studies of differences in distinct cGMP kinase isoforms are highly dependent on expression of large amounts of these enzyme isoforms that are not easily purified by conventional methods. Here cGMP-dependent protein kinases, the type I beta soluble form from

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