P3627
酸性磷酸酶 来源于小麦胚芽
≥0.4 unit/mg solid
别名:
酸性磷酸酶 来源于小麦胚芽, （正磷酸单酯磷酸水解酶[酸最适]）
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Analysis Note
含有脂肪酶
Application
酸性磷酸酶（APase）可非特异性地催化磷酸单酯和酸酐的水解以产生无机磷酸盐。它可用于研究磷酸盐的生产、转运和再循环，以及细胞的代谢和能量转导过程。 产品P3627来自于小麦胚芽，已被用于确定磷酸酶处理对3F3/2染色的影响。
Biochem/physiol Actions
植物酸性磷酸酶（APase）位于细胞溶质、液泡和细胞壁中。植物酸性磷酸酶对于磷酸盐的获取非常重要，其可以调动土壤中的有机磷酸盐。 磷酸盐饥饿可诱导酸性磷酸酶的产生。 酸性磷酸酶起源于发芽过程中的糊粉和黄芩组织。 它可以水解植酸、ATP、蛋白质磷酸和核苷酸磷酸。 它可在一般代谢反应中起作用。 小麦胚胎APase由四种同工酶组成。 小麦胚芽APase的分子量为58 kDa（凝胶过滤），最适pH值为5.7，pH范围为4.0-7.0，最适温度为45°C。
Other Notes
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
Preparation Note
Prepared essentially by method of Singer, T.P., J. Biol.Chem., 174, 11 (1948).
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
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Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
Kinsley C French et al.
Biochemistry, 51(51), 10127-10136 (2012-12-12)
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Clinical orthopaedics and related research, 471(4), 1365-1372 (2012-12-15)
With bone resorption rates greater than formation, stress fracture pathogenesis plausibly involves bone remodeling imbalance. If this is the case, one would anticipate serum levels of bone turnover markers would be higher in patients with stress fractures than in those
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
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