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主要文件

安全信息

P3476

Millipore

蛋白质 A -琼脂糖fast flow

50%, aqueous suspension

别名:

Protein A resin

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选择尺寸

5 G
¥410.31
25 G
¥553.20
100 G
¥615.51
1 KG
¥898.12

¥410.31


国内现货,预计发货时间2025年4月27日详情



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变更视图
5 G
¥410.31
25 G
¥553.20
100 G
¥615.51
1 KG
¥898.12

About This Item

MDL编号:
UNSPSC代码:
41106500
NACRES:
NA.56

¥410.31


国内现货,预计发货时间2025年4月27日详情


生物来源

protein A from Staphylococcus aureus

描述

protein A leaching <= 5 ng/mL

表单

aqueous suspension

浓度

50%

基质

6% Fast-flow beaded agarose

容量

37-43 mg/mL binding capacity (IgG)

储存温度

2-8°C

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此商品
M6688A7472P9740
technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

aqueous suspension

form

suspension

form

suspension

form

suspension

application(s)

cell analysis

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

composition

solids, 10%

composition

-

composition

Suspension in 60% saturated Ammonium Sulfate and 50 mM Potassium Phosphate, pH 7.0

composition

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

mean particle size

0.24 μm

mean particle size

-

mean particle size

-

mean particle size

-

应用

Protein A琼脂糖用于亲和层析、抗体纯化、表征,以及蛋白 A、G 和 L 树脂。Protein A琼脂糖已用于研究蛋白 A 免疫吸附在慢性扩张型心肌病患者中的作用,以及研究多发性硬化和癌症。
Protein A– 琼脂Fast Flow 已用于:

  • 免疫沉淀转染病毒患者正常肾脏和幼鼠肾脏细胞的蛋白质复合物 TRRAP/p400 的[1]
  • 从抗血清中纯化多克隆 IgG[2]
  • 在 HA-Ano9 质粒转染的人胚胎肾细胞的免疫沉淀分析中[3]

特点和优势

  • 高容量
  • 低泄漏
  • 用于纯化单克隆抗体和多克隆抗体的稳定亲和介质
  • 非常适合从细胞培养中快速捕获免疫球蛋白

外形

悬浮液含 0.01% 硫柳汞

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    Verena K Hehle et al.
    BMC biotechnology, 11, 128-128 (2012-01-03)
    Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. In this study, we investigated the
    Henrik Buschmann et al.
    Journal of cell science, 128(11), 2033-2046 (2015-04-25)
    The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast
    Lu Chen et al.
    STAR protocols, 2(2), 100477-100477 (2021-05-18)
    The majority of the mammalian genome is transcribed into non-coding RNAs, many of which co-evolve with RNA-binding proteins (RBPs) to function as biochemically defined and tractable ribonucleoproteins (RNPs). Here, we applied icSHAPE- a robust and versatile RNA structural probing pipeline-
    Nolan J Hoffman et al.
    Molecular metabolism, 41, 101048-101048 (2020-07-02)
    Glycogen is a major energy reserve in liver and skeletal muscle. The master metabolic regulator AMP-activated protein kinase (AMPK) associates with glycogen via its regulatory β subunit carbohydrate-binding module (CBM). However, the physiological role of AMPK-glycogen binding in energy homeostasis
    Spiro Marangoudakis et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(30), 10365-10369 (2012-07-28)
    Ca(V)2.2 (N-type) calcium channels control the entry of calcium into neurons to regulate essential functions but most notably presynaptic transmitter release. Ca(V)2.2 channel expression levels are precisely controlled, but we know little of the cellular mechanisms involved. The ubiquitin proteasome

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    实验方案

    A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

    SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.

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