推荐产品
形式
lyophilized powder
质量水平
包装
glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g
储存条件
do not freeze
标记范围
3 mg per mL
技术
affinity chromatography: suitable
颜色
white to gray
基质
Sepharose CL-4B
基质活化
cyanogen bromide
基质附着
amino
基质隔离区
1 atom
容量
≥16 mg/mL binding capacity (human IgG)
溶解性
water: 1 g/L at 20 °C
适用性
conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
储存温度
2-8°C
正在寻找类似产品? 访问 产品对比指南
一般描述
应用
- 用于免疫沉淀胎盘,使用蛋白印迹技术进行蛋白质和RNA分析。
- 用于染色质免疫沉淀,以了解介体和特定启动子染色质结构重构之间的联系。
- 用作裂解缓冲液,以去除雌性大鼠脑样本蛋白质提取中的内源性G型Ig。
- 用于兔抗血清的[32P]磷肉豆蔻素C的免疫沉淀反应,以研究蛋白激酶C (PKC)和磷肉豆蔻素C (PMC)在T细胞发育过程中的下调。
数量
外形
法律信息
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
How do I swell P3391, Protein A-Sepharose® resin?
The lyophilized product should be swollen in buffer A for 30 minutes or longer at room temperature. Do not stir with any kind of mechanical stirrer. One gram of powder typically swells to 3-4 mL of the hydrated gel. This, along with other useful information (such as the formulation of buffer A) can be found on the product information sheet (under Documents, above).
How can I store the hydrated P3391, Protein A-Sepharose® resin?
Suspensions stored in the refrigerator in the presence of a suitable bacteriostatic agent, such as 20% ETOH, retain IgG binding activity. Another storage option is to put the resin into phosphate buffer saline, pH 7 with about 0.01% preservative (thimerosal or sodium azide).
What can P3391, Protein A-Sepharose® resin tolerate?
Protein A Sepharose CL-4B exhibits high chemical and mechanical stability, and is stable over the pH range 2-11. It withstands high concentrations of denaturants, such as urea and guanidine hydrochloride. The gel displays high thermal stability but is not autoclavable. Furthermore, the gel can, if handled with care, be used many times.
How do I clean Product P3391, Protein A-Sepharose®, resin?
It may be possible to clean the resin by washing it with 10-20 volumes of 100 mM Tris or borate buffer, pH8.5, containing 0.5-2.0 M NaCl, followed by 10-20 volumes of 100 mM acetate buffer, pH 4.0, containing0.5-2.0 M NaCl. Re equilibrate the resin with 20 volumes of buffer A.
What is the binding capacity of Product P3391, Protein A-Sepharose®, resin for my immunoglobulins?
A table is available on the product information sheet (under Documents, above).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门