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Agency
meets USP testing specifications
质量水平
检测方案
≥92% (chromatography)
90.0-110.0% dry basis
形式
solid
分子量
Mr 5000-10000
技术
transduction: suitable
运输
ambient
储存温度
2-8°C
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一般描述
硫酸鱼精蛋白是阳离子多肽，从鲑鱼精子中分离得到，分子量5 kDa。
应用
硫酸鱼精蛋白用于：
- 将慢病毒转导进人胚胎293T细胞
- 将 shRNA转导入间充质干细胞/骨髓基质细胞（MSC）
- 将 慢病毒Sca1-OCT4质粒转导入人脐血造血祖细胞CD34+ 细胞
生化/生理作用
碱性鱼精蛋白与带负电荷的肝素结合。在心血管外科手术中使用鱼精蛋白有时会引发过敏反应。
鱼精蛋白有助于使用阳离子脂质体直接转染微生物 细胞和人类细胞。鱼精蛋白能够有效中和肝素的抗凝作用。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
shRNA-mediated decreases in c-Met levels affect the differentiation potential of human mesenchymal stem cells and reduce their capacity for tissue repair
Rosova I, et al.
Tissue Engineering: Part A, 16(8), 2627-2639 (2010)
Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation
Ainle FN, et al.
Blood, 114(8), 1658-1665 (2009)
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