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重组
expressed in E. coli
质量水平
表单
aqueous ethanol suspension
分析物化学类别
proteins (Immunoglobulins of various mammalian species)
标记范围
~2 mg per mL
技术
affinity chromatography: suitable
基质
Sepharose 4B Fast Flow
基质活化
cyanogen bromide
基质附着
amino
基质隔离区
1 atom
储存温度
2-8°C
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一般描述
蛋白G是从G组链球菌菌株G-148中分离得到的细菌胞壁蛋白，它能与免疫球蛋白（IgG）结合。通过木瓜蛋白酶消化从细胞中提取该蛋白，并依次使用DEAE-Sephadex离子交换层析、琼脂糖凝胶偶联人IgG亲和层析以及Sephadex G-200凝胶层析进行纯化。蛋白G与各种多克隆和单克隆IgG结合的pH条件基本在2.8-10之间，在pH 4-5时结合最强，在pH 10时结合最弱。它是强大的IgG检测试剂。
蛋白G是从G组链球菌菌株G-148中分离得到的细菌胞壁蛋白，它能与免疫球蛋白（IgG）结合。通过木瓜蛋白酶消化从细胞中提取该蛋白，并依次使用DEAE-Sephadex离子交换层析、琼脂糖凝胶偶联人IgG亲和层析以及Sephadex G-200凝胶层析进行纯化。蛋白G与各种多克隆和单克隆IgG结合的pH条件基本在2.8-10之间，在pH 4-5时结合最强，在pH 10时结合最弱。它是强大的IgG检测试剂。
P3296-5Ml的最新产品编号为GE17-0618-01
P3296-5Ml的最新产品编号为GE17-0618-01
应用
蛋白G琼脂糖凝胶™ 被用于亲和层析，蛋白质层析，抗体纯化和表征，免疫亲和基质，蛋白A、G和L树脂，蛋白质相互作用以及纯化和检测。蛋白G琼脂糖凝胶™ 已被用于开发一种可确认人血清中是否存在抗促红细胞生成素中和抗体的方法，以及比较马血清中白蛋白和IgG消耗的方法。
外形
悬浮于20%乙醇中
制备说明
使用重组链球菌蛋白G制备，其中白蛋白结合区已被移除
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
115.0 °F - closed cup
闪点(°C)
46.1 °C - closed cup
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
G Yang et al.
Oncogene, 26(1), 91-101 (2006-06-27)
The t(8;21) chromosomal translocation that generates the fusion oncoprotein RUNX1-ETO predominates in leukemia patients of the French-American-British (FAB) class M2 subtype. The oncoprotein has the capacity to promote expansion of hematopoietic stem/progenitor cells and induces leukemia in association with other
B Akerström et al.
Journal of immunology (Baltimore, Md. : 1950), 135(4), 2589-2592 (1985-10-01)
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein recently isolated from group G streptococci. We have investigated the avidity of protein G for various monoclonal and polyclonal Ig of the IgG class, and compared it with the binding
Yi-Jye Chern et al.
Cell death & disease, 10(7), 504-504 (2019-06-28)
Therapy-refractory disease is one of the main contributors of treatment failure in cancer. In colorectal cancer (CRC), SPARC can function as a sensitizer to conventional chemotherapy by enhancing apoptosis by interfering with the activity of Bcl-2. Here, we examine a
Aaron Pinnola et al.
The Journal of biological chemistry, 282(44), 32511-32519 (2007-09-11)
Poly(ADP-ribose) polymerase 1 protein (PARP1) mediates chromatin loosening and activates the transcription of inducible genes, but the mechanism of PARP1 regulation in chromatin is poorly understood. We have found that PARP1 interaction with chromatin is dynamic and that PARP1 is
B Akerström et al.
The Journal of biological chemistry, 261(22), 10240-10247 (1986-08-05)
Protein G, an IgG-binding molecule, was prepared from the cell walls of a group G streptococcal strain, G-148. The protein could be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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