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安全信息

P3223

Sigma-Aldrich

(−29) M13 反向引物组

别名:

5'-CAG GAA ACA GCT ATG ACC-3'

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About This Item

UNSPSC代码:
41106305

储存温度

2-8°C

应用

(-29) M13 反向引物组是单链寡核苷酸,5′-羟基和 3′-羟基末端和用于聚合酶链反应方案 (PCR) 的四个荧光标签的选择。该引物组经过功能测试,可用于荧光检测自动测序。

包装

采用琥珀色管包装,防止光线照射。

组分

一组四瓶引物,每个引物在5 ′ 末端单独标记有:
FAM:(5 [6]-羧基荧光素)
JOE: (6-羧基-4′,5′-二氯-2′,7′-二甲氧基荧光素)
ROX:(5 [6]-羧基-X-罗丹明),以及
TAMRA:(5 [6]-羧基四甲基罗丹明)。
以溶液形式提供,每份 50μl,溶于 pH 7.5 tris-EDTA 缓冲液中,浓度为 1 皮摩尔/μl

分析说明

纯度已通过HPLC、PAGE和OD进行测试。

储存分类代码

10 - Combustible liquids

法规信息

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High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
K A Abd-Elsalam et al.
Genetics and molecular research : GMR, 9(4), 2016-2024 (2010-10-20)
Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed
Yajing Zhou et al.
PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the
Angela Capece et al.
International journal of food microbiology, 144(1), 187-192 (2010-10-12)
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae

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