P2645
蛋白激酶 A 催化亚基 来源于牛心脏
≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
别名:
PKA
产品名称
蛋白激酶 A 催化亚基 来源于牛心脏, ≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
form
lyophilized (white powder to sticky mass to hard pellet)
specific activity
≥9 units/μg protein (cyclic-AMP is not required for this activity)
mol wt
40,862 Da
storage temp.
−20°C
Quality Level
Application
牛心脏蛋白激酶A （PKA）催化亚单位已用于
- 研究PKA介导的IRK1（内向整流K+）通道抑制
- 体外 PKA磷酸化测定
- 体外亲和结合测定
- 研究PKA对功能活动运动神经元吸气驱动电流的影响
Biochem/physiol Actions
蛋白激酶A (PKA)控制Hedgehog信号转导，并参与增殖和命运决定。它可以使几种神经递质受体、转录因子和多种细胞内信号通路的成分磷酸化。
蛋白激酶A催化末端磷酸从ATP转移到存在于各种蛋白质上的苏氨酸或丝氨酸残基。这种蛋白质在没有环磷酸腺苷的情况下是无活性的，环磷酸腺苷是催化亚基和调节亚基结合在一起的地方。存在cAMP时，调节亚单位与cAMP结合并释放催化亚单位。
Disclaimer
请注意为了与单位定义保持一致我们修改了包装尺寸，但是磷酸化单位的数量和以前相同。
General description
蛋白激酶一种酶由两个亚单位（催化亚单位和调节亚单位）组成。催化亚基在环磷酸腺苷存在下作为单体存在，分子量为40,862 Da。
Other Notes
磷酸化活性： 在 pH 6.5、30°C，一单位每分钟可将 1.0 pmol磷酸盐从 ATP转化为水解和部分去磷酸化的酪蛋白，并可通过测量ADP产量进行测定。
Packaging
包装规格基于磷酸化单位
Physical form
冻干粉，含蔗糖和磷酸缓冲盐作为稳定剂。
Preparation Note
由蛋白激酶 A (P 5511) 制成
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Ken Tougane et al.
Plant physiology, 152(3), 1529-1543 (2010-01-26)
Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the
Cyclic nucleotides in the nervous system
Basic Neurochemistry, 423-441 (2012)
Yoshiko Iida et al.
European journal of biochemistry, 269(19), 4780-4788 (2002-10-02)
We previously demonstrated in mast cell lines RBL2H3 and FMA3 that tryptophan hydroxylase (TPH) undergoes very fast turnover driven by 26S-proteasomes [Kojima, M., Oguro, K., Sawabe, K., Iida, Y., Ikeda, R., Yamashita, A., Nakanishi, N. & Hasegawa, H. (2000) J.
Christopher M Bocchiaro et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(4), 1099-1103 (2003-02-25)
Plasticity underlying adaptive, long-term changes in breathing behavior is hypothesized to be attributable to the modulation of respiratory motoneurons by intracellular second-messenger cascades. In quiescent preparations, protein kinases, including cAMP-dependent protein kinase A (PKA), potentiate glutamatergic inputs. However, the dynamic
E Wischmeyer et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5819-5823 (1996-06-11)
Strongly rectifying IRK-type inwardly rectifying K+ channels are involved in the control of neuronal excitability in the mammalian brain. Whole-cell patch-clamp experiments show that cloned rat IRK1 (Kir 2.1) channels, when heterologously expressed in mammalian COS-7 cells, are inhibited following
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