产品名称
蛋白 A-琼脂糖, saline suspension
biological source
Staphylococcus aureus
form
saline suspension
extent of labeling
~2 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥15 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
Quality Level
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Application
Protein A-agarose 用于亲和层析、抗体纯化和表征以及 Protein A、G 和 L 树脂。蛋Protein A-agarose 已被用于研究慢性扩张型心肌病患者的蛋白 A 免疫吸附作用以及研究多发性硬化和胃癌。
蛋白 A-琼脂糖已用于小鼠成纤维细胞 L-cell 和人胚肾 (HEK) 293 细胞中 Wnt3a 和音猬因子 (Shh) 的脯氨酰羟化酶结构域 2 N27 细胞的 S63 和 NIH3T3 成纤维细胞的蛋白磷酸酶和张力蛋白同源物 (PTEN) 蛋白的
- 免疫沉淀
Biochem/physiol Actions
蛋白 A 与 Fcγ（恒定区）以及 IgG 的 Fab（抗原结合片段）结合。蛋白 A 琼脂糖树脂在商业上用于基于亲和力的纯化。
General description
蛋白 A 是来自 金黄色葡萄球菌 的 42-kDa 蛋白，具有 5 个免疫球蛋白结合域。
Physical form
混悬于含防腐剂的0.5 M NaCl中
存储类别
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes
Gao X, et al.
Febs Letters, 585(15), 2501-2506 (2011)
D J McCool et al.
The Biochemical journal, 312 ( Pt 1), 125-133 (1995-11-15)
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete MUC2 without the addition of secretagogues. Treatment of the LS180 cells with
D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
Zeleniak AE, et al.
Neoplasia, 20(1), 12-24 (2018)
Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins
McCaw TR, et al.
Biotechnology Progress, 30(5), 1125-1136 (2014)
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