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Merck
CN

P2545

蛋白 A-琼脂糖

saline suspension

别名:

Protein A resin

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关于此项目

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
saline suspension
Biological source:
Staphylococcus aureus
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biological source

Staphylococcus aureus

Quality Level

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

General description

蛋白 A 是来自 金黄色葡萄球菌 的 42-kDa 蛋白,具有 5 个免疫球蛋白结合域。

Application

蛋白 A-琼脂糖已用于小鼠成纤维细胞 L-cell 和人胚肾 (HEK) 293 细胞中 Wnt3a 和音猬因子 (Shh) 的脯氨酰羟化酶结构域 2 N27 细胞的 S63 和 NIH3T3 成纤维细胞的蛋白磷酸酶和张力蛋白同源物 (PTEN) 蛋白的
  • 免疫沉淀

Protein A-agarose 用于亲和层析、抗体纯化和表征以及 Protein A、G 和 L 树脂。蛋Protein A-agarose 已被用于研究慢性扩张型心肌病患者的蛋白 A 免疫吸附作用以及研究多发性硬化和胃癌。

Biochem/physiol Actions

蛋白 A 与 Fcγ(恒定区)以及 IgG 的 Fab(抗原结合片段)结合。蛋白 A 琼脂糖树脂在商业上用于基于亲和力的纯化。

Physical form

混悬于含防腐剂的0.5 M NaCl中


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存储类别

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

法规信息

常规特殊物品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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相关内容

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001


D J McCool et al.
The Biochemical journal, 302 ( Pt 1), 111-118 (1994-08-15)
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each
PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
Zeleniak AE, et al.
Neoplasia, 20(1), 12-24 (2018)
D J McCool et al.
The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin



全球贸易项目编号

货号GTIN
P2545-5ML04061834364009
P2545-1ML04061834363996