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Merck
CN

P2143

Sigma-Aldrich

蛋白酶 来源于佐氏曲霉

Type XIII, ≥0.6 unit/mg solid

别名:

Molsin

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

Aspergillus sp. (Aspergillus saitoi)

质量水平

类型

Type XIII

形式

solid

比活

≥0.6 unit/mg solid

异质活性

alkaline protease, essentially free

储存温度

−20°C

相关类别

应用

Aspergillus saitoi来源蛋白酶已用于一项评估大蛋白氢交换序列覆盖率和分辨率的研究中。它还被用于一项通过β-葡萄糖苷酶研究大豆凝乳异黄酮糖苷向其糖苷配基转化的研究中。

生化/生理作用

Aspergillus saitoi来源蛋白酶也可发挥β-葡萄糖苷酶的作用。

单位定义

在pH 2.8、37℃条件下,一单位每分钟可水解酪蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色(使用Folin-Ciocalteu试剂显色)。

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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R E Cardoza et al.
Biotechnology and bioengineering, 83(3), 249-259 (2003-06-05)
A copy of the bovine chymosin gene (chy) with a codon usage optimized for its expression in Aspergillus awamori was constructed starting from synthetic oligonucleotides. To study the ability of this filamentous fungus to secrete bovine prochymosin, two plasmids were
E Skyttä et al.
The Journal of applied bacteriology, 74(2), 134-142 (1993-02-01)
The broad-spectrum antibacterial activity exhibited by three Pediococcus strains isolated from beer was preliminarily characterized. Factors affecting the production rate of bacterial inhibitors were screened and the effects of simultaneous cultivation of Lactococcus and Pediococcus on the production of inhibitory
E Ichishima
Bioscience, biotechnology, and biochemistry, 64(4), 675-688 (2000-06-01)
This review covers the unique catalytic and molecular properties of three proteolytic enzymes and a glycosidase from Aspergillus. An aspartic proteinase from A. saitoi, aspergillopepsin I (EC 3.4.23.18), favors hydrophobic amino acids at P1 and P'1 like gastric pepsin. However
F J Moralejo et al.
Applied microbiology and biotechnology, 54(6), 772-777 (2001-01-11)
A gene encoding the sweet-tasting protein thaumatin (tha) with optimized codon usage was expressed in Aspergillus awamori. Mutants of A. awamori with reduced proteolytic activity were isolated. One of these mutants, named lpr66, contained an insertion of about 200 bp
P J Roubos-van den Hil et al.
Journal of applied microbiology, 109(1), 137-145 (2009-12-17)
Tempe, a Rhizopus ssp.-fermented soya bean food product, was investigated for bacteriostatic and/or bactericidal effects against cells and spores of the food-borne pathogen Bacillus cereus. Tempe extract showed a high antibacterial activity against B. cereus ATCC 14579 based on optical

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