等级
for molecular biology
质量水平
检测方案
≥99.0%
形式
liquid
pH值（酸碱度）
4.3-4.7
应用
life science and biopharma
储存温度
2-8°C
一般描述
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.
应用
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
- for the extraction and purification of RNA.
- to isolate RNA from Arabidopsis siliques.
- to extract proteins and DNA from polysomal and non-polysomal fractions.
- in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
靶器官
Liver,Kidney, Nervous system,Kidney,Liver,Skin
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
新产品
PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
Molecular approaches for analysis of Drosophila MicroRNAs
Pandey M, et al.
Journal of Separation Science, 169-188 (2020)
Exploring translation regulation by global analysis of ribosomal association
Melamed D, et al.
Methods in Enzymology, 48(3), 301-305 (2009)
Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds
Berardino JD, et al.
Journal of Experimental Botany, 69(6), 1403-1414 (2018)
商品
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
了解 mRNA 疫苗如何诱导免疫，以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。
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