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Merck
CN

P1944

苯酚/氯仿/异戊醇（125:24:1）

Molecular Biology

别名:

phenol chloroform RNA extraction

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关于此项目

NACRES:
NA.52
UNSPSC Code:
12190000
Grade:
Molecular Biology
Assay:
≥99.0%
Application(s):
life science and biopharma

主要文件

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产品名称

苯酚/氯仿/异戊醇（125:24:1）, Molecular Biology

grade

Molecular Biology

assay

≥99.0%

form

liquid

pH

4.3-4.7

application(s)

life science and biopharma

storage temp.

2-8°C

Quality Level

200

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

target_organs

Liver,Kidney, Nervous system,Kidney,Liver,Skin

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

175.0 °F - closed cup

flash_point_c

79.44 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCZ9459
MKCZ6246
MKCZ6245
MKCX5083
MKCW7584

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PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C Puissant et al.
BioTechniques, 8(2), 148-149 (1990-02-01)
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
C Pistore et al.
Oncogene, 36(40), 5551-5566 (2017-06-06)
Widespread genome hypo-methylation and promoter hyper-methylation of epithelium-specific genes are hallmarks of stable epithelial-to-mesenchymal transition (EMT), which in prostate cancer (PCa) correlates with castration resistance, cancer stem cells generation, chemoresistance and worst prognosis. Exploiting our consolidated 'ex-vivo' system, we show
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