P1484
抗-p21WAF1/Cip1抗体，小鼠单克隆 小鼠抗
clone CP74, purified from hybridoma cell culture
别名:
抗-CDKN1A, 抗-Cip1, 抗-WAF1, 抗-p21
产品名称
抗-p21WAF1/Cip1抗体，小鼠单克隆 小鼠抗, clone CP74, purified from hybridoma cell culture
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CP74, monoclonal
form
buffered aqueous solution
mol wt
antigen 21 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)
General description
小鼠单克隆克隆CP74抗p21WAF1/Cip1抗体可与p21WAF1/Cip1发生特异性反应。两个CKI家族已得到鉴定。p21WAF1/Cip1家族包含p21WAF1/Cip1、p27Kip1和p57Kip2。
单克隆抗p21WAF1/Cip1（小鼠IgG2b同种型）产生于由小鼠骨髓瘤细胞与人重组p21WAF1/Cip1免疫的BALB/c小鼠脾细胞融合后而产生的CP74杂交瘤细胞。p21WAF1/Cip1大小为21 kDa，也被称为Cip1、WAF1、Sdi1、Pic1、CAP20。
Immunogen
重组人p21WAF1/Cip1。
Physical form
溶于磷酸盐缓冲液，pH 7.4，含有1%牛血清白蛋白以及15 mM叠氮化钠
Application
小鼠单克隆克隆CP74抗p21WAF1/Cip1抗体可用于ELISA、免疫印迹（21 kDa）和免疫沉淀（p21/细胞周期蛋白A/Cdk2复合物，天然和变性的）。存在有能够与p21WAF1/Cip1发生反应的单克隆抗体，使得对p21WAF1/Cip1进行亚细胞检测和定位以及测量p21WAF1/Cip1水平的相对差异作为细胞周期阶段的函数成为可能。
Biochem/physiol Actions
p21WAF1/Cip1可抑制所有参与到细胞周期中G1/S过渡的激酶。p21WAF1/Cip1能与G1和S期激酶、细胞周期蛋白E/Cdk2、细胞周期蛋白D/Cdk4和细胞周期蛋白A/Cdk2紧密结合并有效抑制它们的活性，而p21WAF1/Cip1对G2/M期激酶细胞周期蛋白B/Cdc2的抑制作用则相对较弱。此外，p21WAF1/Cip1抑制增殖细胞核抗原（PCNA），结合并抑制Rb的失活，这对于细胞周期进程至关重要，并且还可能与E2F相互作用并保护细胞免受p53介导的细胞凋亡。
真核细胞中细胞周期进展的调节取决于细胞周期蛋白这类蛋白的表达。这些蛋白可与多种不同的细胞周期蛋白依赖性激酶（CDK）形成复合物。 细胞周期蛋白依赖性激酶（CDK）的催化活性受两种通用机制调节：蛋白磷酸化以及与包括细胞周期蛋白和CDK抑制剂（CKI）等调节亚基的结合。它们可对所有参与到G1/S过渡的激酶进行抑制。包括p15INK4b、p16INK4a、p18INK4c和p19INK4d的p16INK4a家族可特异性抑制Cdk4和Cdk6。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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