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形式
ammonium sulfate suspension
质量水平
比活
≥10.0 units/mg protein (modified Warburg-Christian)
secondary activity
≤9.9 units/mg protein ATPase
分子量
110 kDa
储存温度
2-8°C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
一般描述
甘薯酸性磷酸酶是一种磷酸单酯酶，具有多种分子形式，分子量相近，但等电点不同。
应用
在一项研究中，利用甘薯酸性磷酸酶与戊二醛和氨丙基可控孔径玻璃共价结合后，可用作柱前酶反应器。它还被用于研究酪氨酸、组氨酸和半胱氨酸作为甘薯磷酸酶中Mn(III)的配体的用途。
甘薯酸性磷酸酶被作为去磷酸化试剂用于硫色素测定，以检测生物样品中的硫胺。在该测定中，该酶比小麦胚芽酸性磷酸酶或α-淀粉酶更有效。
生化/生理作用
酸性磷酸酶（APase）是一个酶家族，在pH 4-7最佳条件下，通过以下反应非特异性地催化磷酸单酯和酸酐水解，产生无机磷酸盐： APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+它们在磷酸盐生成、转运和再循环中的作用对于细胞的代谢和能量转导过程至关重要。Apase作为一类酶，可能与调节过程中的激酶具有同等重要的作用。
单位定义
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
外形
悬浮于1.8 M (NH4)2SO4，10 mM MgCl2，pH 5.3
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
G Schenk et al.
Archives of biochemistry and biophysics, 370(2), 183-189 (1999-10-08)
Purple acid phosphatases comprise a family of binuclear metal-containing acid hydrolases, representatives of which have been found in animals, plants, and fungi. The goal of this study was to characterize purple acid phosphatases from sweet potato tubers and soybean seeds
Susumu Yamato et al.
Biological & pharmaceutical bulletin, 27(2), 210-215 (2004-02-06)
Sweet potato acid phosphatase was covalently coupled with glutaraldehyde to aminopropyl controlled-pore glass, and used as a pre-column enzyme reactor. The immobilized enzyme reactor (IMER) was continuously operated using an automated chromatographic detection system we developed. Functional evaluation of the
An unstable manganese(III) complex incorporating ligand donor types proposed for an acid phosphatase from sweet potato: (p-nitrobenzenethiolato)[N,N'-ethylenebis(salicylideneaminato)]manganese(III)
Gohdes, J. and W. Armstrong
Inorganic Chemistry, 27, 1841-1842 (1988)
D T Wyatt et al.
Clinical chemistry, 35(11), 2173-2178 (1989-11-01)
We analyzed extensively a modified thiochrome method for thiamin analysis. Acid phosphatase (EC 3.1.3.2) from potato was superior to either alpha-amylase or acid phosphatase from wheat germ as a dephosphorylating agent. Timing of cyanogen bromide exposure was important, but the
Mina Solhtalab et al.
Environmental science & technology, 56(22), 16441-16452 (2022-10-26)
Among ubiquitous phosphorus (P) reserves in environmental matrices are ribonucleic acid (RNA) and polyphosphate (polyP), which are, respectively, organic and inorganic P-containing biopolymers. Relevant to P recycling from these biopolymers, much remains unknown about the kinetics and mechanisms of different
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以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
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