P1146
酸性磷酸酶 来源于马铃薯
lyophilized powder, ≥3.0 units/mg solid
别名:
(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Specific activity:
≥3.0 units/mg solid
form
lyophilized powder
Quality Level
specific activity
≥3.0 units/mg solid
mol wt
69 kDa
shipped in
wet ice
storage temp.
−20°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
General description
马铃薯酸性磷酸酶是一种磷酸单酯酶，具有多种分子形式，分子量相近，但等电点不同。
Application
检测了马铃薯酸性磷酸酶在各种表面活性剂介质中的活性。 双（2-乙基己基）磺基琥珀酸钠，Brij 35和3mM的SDS抑制磷酸酶活性，而表面活性剂十六烷基三甲基溴化铵增强活性。
马铃薯酸性磷酸酶已用于评估新基因产物的潜在过敏性的研究中。 在一项研究中，它还用于在pH 7.0下从酪蛋白中去除8个磷酸基团。
Biochem/physiol Actions
酸性磷酸酶（APase）是一个酶家族，在pH 4-7最佳条件下，非特异性地催化磷酸单酯和酸酐水解产生无机磷酸盐。
Other Notes
在pH 4.8 ，37℃ 下，一单位每分钟可水解 1.0 μmole的对-硝基苯基磷酸酯。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
相关内容
J Lalitha et al.
Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)
The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3
Mariusz Olczak et al.
Acta biochimica Polonica, 50(4), 1245-1256 (2004-01-24)
The properties of plant purple acid phosphatases (PAPs), metallophosphoesterases present in some bacteria, plants and animals are reviewed. All members of this group contain a characteristic set of seven amino-acid residues involved in metal ligation. Animal PAPs contain a binuclear
Y Sugiura et al.
The Journal of biological chemistry, 256(20), 10664-10670 (1981-10-25)
A new manganese-containing acid phosphatase has been isolated and crystallized from sweet potato tubers. The pure enzyme contains one atom of manganese per Mr = 110,000 polypeptide and shows phosphatase activity toward various phosphate substrates. The pH optimum of the
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P1146-50UN | 04061834354109 |
| P1146-1KU | 04061834354086 |
| P1146-200UN | 04061834354093 |