P1146
酸性磷酸酶 来源于马铃薯
lyophilized powder, ≥3.0 units/mg solid
别名:
(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
产品名称
酸性磷酸酶 来源于马铃薯, lyophilized powder, ≥3.0 units/mg solid
SMILES string
O(CC(O)C)CC#C
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
form
lyophilized powder
specific activity
≥3.0 units/mg solid
mol wt
69 kDa
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
检测了马铃薯酸性磷酸酶在各种表面活性剂介质中的活性。 双（2-乙基己基）磺基琥珀酸钠，Brij 35和3mM的SDS抑制磷酸酶活性，而表面活性剂十六烷基三甲基溴化铵增强活性。
马铃薯酸性磷酸酶已用于评估新基因产物的潜在过敏性的研究中。 在一项研究中，它还用于在pH 7.0下从酪蛋白中去除8个磷酸基团。
Biochem/physiol Actions
酸性磷酸酶（APase）是一个酶家族，在pH 4-7最佳条件下，非特异性地催化磷酸单酯和酸酐水解产生无机磷酸盐。
General description
马铃薯酸性磷酸酶是一种磷酸单酯酶，具有多种分子形式，分子量相近，但等电点不同。
Other Notes
在pH 4.8 ，37℃ 下，一单位每分钟可水解 1.0 μmole的对-硝基苯基磷酸酯。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
J Lalitha et al.
Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)
The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3
S I Tu et al.
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
P P Waymack et al.
Archives of biochemistry and biophysics, 288(2), 621-633 (1991-08-01)
An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated.
Sishuo Cao et al.
Regulatory toxicology and pharmacology : RTP, 63(2), 181-187 (2012-04-17)
With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three
Y Sugiura et al.
The Journal of biological chemistry, 256(20), 10664-10670 (1981-10-25)
A new manganese-containing acid phosphatase has been isolated and crystallized from sweet potato tubers. The pure enzyme contains one atom of manganese per Mr = 110,000 polypeptide and shows phosphatase activity toward various phosphate substrates. The pH optimum of the
实验方案
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
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