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一般描述
来自小牛肠的碱性磷酸酶是一种糖蛋白，分子量为140,000。
应用
小牛肠道碱性磷酸酶（CIAP）已用于通过分光光度法评估丹磺酰基-L-苯丙氨酸的抑制作用。 CIAP还被证明能够清除脂多糖（LPS）介导的炎症反应，这在治疗LPS介导的疾病中可能具有重要作用。
来自小牛肠的碱性磷酸酶CIP可去除DNA的5′末端的磷酸基团，有助于克隆技术，防止DNA自连接。它可以被高精氨酸抑制。
生化/生理作用
Sigma不溶性酶是通过使用常规“可溶”酶与惰性基质反应，获得保留原始酶活性的不溶性化合物而生成的。应用几乎不受限制。通过仔细选择“惰性”基质，可产生高活性的“树脂”或“凝胶”，它们与底物在合适介质中的瞬时接触，能够迅速催化酶反应。反应可以在间歇桨液反应器中进行，甚至可以在小柱中进行。无论是哪种情况，不溶性酶都不会污染反应混合物，可以很容易地将其从间歇桨液中滤出或留在色谱柱中。许多不稳定酶在不溶形式下可能会更加稳定。
包装
包装规格取决于 DEA单位
单位定义
一个酶活性单位是指在pH 9.8、37°C下，每分钟水解1.0 mmole 对硝基苯磷酸盐所需的酶量。
外形
悬浮于 2.0 M (NH4)2SO4，1 mM MgCl2，0.1 mM ZnCl2，pH 7
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
A Bakker et al.
Journal of virology, 70(8), 5511-5518 (1996-08-01)
The Rex protein is an essential regulator of RNA expression in human T-cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2) that promotes the accumulation of full-length and partially spliced viral transcripts in the cytoplasm. Rex-mediated regulation correlates with
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least
Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets
Beumer, C.
Journal of Pharmacology and Experimental Therapeutics, 397, 737-744 (2003)
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