生物来源
Porcine gastric mucosa
质量水平
形式
lyophilized powder
比活
≥30 units/mg dry solid
分子量
35 kDa
基质
4% cross-linked beaded agarose
UniProt登记号
储存温度
−20°C
基因信息
pig ... LOC396892(396892)
相关类别
一般描述
胃蛋白酶是一种在胃液分泌物中发现的酶。
应用
固定在琼脂糖珠上的胃蛋白酶可用于消化并随后从糖蛋白麦醇溶蛋白中分离出活性肽。
用于产生抗体的F(ab′)2 片段。1
胃蛋白酶-琼脂糖已作为固定工具用于研究胃蛋白酶的重折叠能力。它还用于从兔IgG制备F(ab′)2片段。
生化/生理作用
优先将C末端切割成Phe、Leu和Glu。它不会在Val、Ala或Gly上进行切割。最佳pH2-4。活性成分为4 M尿素和3 M盐酸胍。在60°C时稳定。胃蛋白酶在pH > 6时会不可逆地失活。
单位定义
在pH2.0，37℃条件下用血红蛋白作为底物，对TCA可溶性产物进行测定时，一个单位每分钟可产生0.001 ΔA的280（终体积 = 16 mL。光程 = 1 cm。）
数量
一克固体可膨胀至5-10ml填充凝胶。
外形
用乳糖稳定化的冻干粉
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Widya A Wahab et al.
International journal of experimental pathology, 97(4), 303-309 (2016-09-24)
Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
商品
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
实验方案
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。
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