蛋白激酶抑制剂 来源于猪心脏

These proteins bind to the catalytic subunit of cyclic-AMP dependent protein kinase, displacing the regulatory subunit and inhibiting phosphorylating activity. The cyclic-AMP binding capacity of the regulatory subunit is enhanced. Many other proteins exhibit this enhancement ability, too (Brostrom, C.O. and Kon, C., Anal. Biochem., 58, 459 [1974]).

Package size based on protein content.

Supplied as lyophilized powder containing 30% glycerophosphate and 20% EDTA.

Fractionated essentially by procedure of Walsh, D.A., et al., J. Biol. Chem., 246, 1977 (1971).

One unit will transfer 1.0 picomole (10^-12 mol) of phosphate from γ-³²P-ATP to hydrolyzed and partially dephosphorylated casein (Product No. C 4765) per min at pH 6.5 at 30 °C.