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一般描述
来自蜡状芽孢杆菌的青霉素酶在纯化时显示含有三种不同的物质,并通过沉降分析、氨基末端分析和垂直丙烯酰胺凝胶电泳检查均一性。
应用
用于生产青霉素。
用于生产青霉素。来自蜡状芽孢杆菌的青霉素酶与来自枯草芽孢杆菌的青霉素酶进行了比较研究,其中米氏常数,Ph/活性曲线的形状和免疫学性质显示出显着不同。
青霉素酶可用于降低严重青霉素反应患者的青霉素水平。
生化/生理作用
青霉素酶特异性催化青霉素的 β-内酰胺环的水解。
单位定义
一个单元将在 pH7.0,25℃ 下,每分钟水解 1.0 μmole 指定的底物。以苄青霉素单位出售。该国际单位(使用苄青霉素作为底物)约等于 600 Levy 或 75 Pollock 单位。
外形
含有磷酸盐和柠檬酸盐缓冲盐的冻干粉末
分析说明
通过缩二脲法测定的蛋白质。
抑制剂
警示用语:
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
Chemical science, 13(6), 1746-1758 (2022-03-15)
Large-scale population screening for early and accurate detection of disease is a key objective for future diagnostics. Ideally, diagnostic tests that achieve this goal are also cost-effective, fast and easily adaptable to new diseases with the potential of multiplexing. Mass
Anaphylactic shock after therapy with penicillinase.
Journal of the American Medical Association, 169(6), 593-594 (1959-02-07)
Journal of bioscience and bioengineering, 110(1), 8-11 (2010-06-15)
Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater
Journal of pharmaceutical and biomedical analysis, 8(1), 49-60 (1990-01-01)
An automated assay for the determination of penicillin in formulations suitable for use in pharmaceutical quality control is presented. The method is based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis (FIA) system. The
Properties of penicillinase from Bacillus cereus 569.
The Journal of biological chemistry, 245(9), 2205-2212 (1970-05-10)
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