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Merck
CN

P0091

Sigma-Aldrich

PolyFreeze

water-soluble support matrix

别名:

water-soluble support matrix for frozen sectioning

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About This Item

UNSPSC代码:
12171500
NACRES:
NA.47

质量水平

颜色

colorless

密度

1.030 g/cm3

应用

hematology
histology

储存温度

room temp

一般描述

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

应用

PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples.

制备说明

PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance.

储存及稳定性

Store the product at room temperature

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Soleto I, et al.
Frontiers in Immunology, 9, 59-59 (2018)
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Acta histochemica, 121(4), 460-471 (2019-04-14)
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Autosomal dominantly inherited genetic disorders such as corneal dystrophies are amenable to allele-specific gene silencing with small interfering RNA (siRNA). siRNA delivered to the cornea by injection, although effective, is not suitable for a frequent long-term treatment regimen, whereas topical

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