生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
PF-29, monoclonal
形式
buffered aqueous solution
分子量
antigen ~43 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using NT2/D1 total cell extract
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... POU5F1(5460)
一般描述
Monoclonal Anti-POU5F1 (Oct4) (mouse IgG2a isotype) is derived from the hybridoma PF-29 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a peptide.
POU5F1 (POU class 5 homeobox 1) gene codes for a transcription factor that binds to octamer sequence. POU5F1 is mapped to human chromosome 6p21.33.
特异性
Monoclonal Anti-POU5F1 (Oct4) recognizes human POU5F1/Oct4.
免疫原
amino acids 20-37 of human POU5F1 peptide.
应用
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
生化/生理作用
POU5F1 (POU class 5 homeobox 1) controls homeostasis of embryonic cell development and pluripotency. The POU5F1 gene is highly expressed in cancer cells and offers resistance against chemotherapy.
POU5F1 protein functions as an early developmental control gene. This protein has been recognized as fundamental in the maintenance of pluripotency of embryonic stem cells and primordial germ cells. POU5F1 protein has been proposed as a useful marker for germ cell tumors (GCTs) that can exhibit pluripotential, specifically seminoma/ dysgerminoma/germinoma and embryonal carcinoma (EC). POU5F1 has also been established as a marker for extragonadal germ cell tumors (GCT) and metastatic tumors.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Clinical significance of putative markers of cancer stem cells in gastric cancer: A retrospective cohort study.
Chen X L, et al.
Oncotarget, 7(38), 62049-62049 (2016)
Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer.
Lu C S, et al.
Oncotarget, 8(19), 30844-30844 (2017)
Timothy D Jones et al.
The American journal of surgical pathology, 28(7), 935-940 (2004-06-30)
OCT4 (POU5F1) is a transcription factor expressed in embryonic stem and germ cells and is involved in the regulation and maintenance of pluripotency. It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma.
Ming-Tse Sung et al.
Human pathology, 37(6), 662-667 (2006-05-31)
Correctly diagnosing a metastatic germ cell tumor after chemotherapy may be challenging because of the diverse morphological manifestations of postchemotherapy tumors. Both OCT4 and CD30 are sensitive markers for the identification of primary embryonal carcinomas; however, loss of expression of
Minimum regions of genomic imbalance in stage I testicular embryonal carcinoma and association of 22q loss with relapse.
Gilbert D C, et al.
Genes Chromosomes Cancer, 50(3), 186-195 (2011)
商品
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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