生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~51 kDa
种属反应性
rat (predicted), mouse (predicted), human
浓度
~1 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS
western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer67)
基因信息
human ... PDCD4(27250) , SELP(6403)
mouse ... Pdcd4(18569)
一般描述
Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.
应用
Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.
生化/生理作用
Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
Dorrello NV, et al.
Science (New York, N.Y.), 314(5798), 467-471 (2006)
Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21Waf1/Cip1
Goke R, et al.
American Journal of Physiology. Cell Physiology, C1541-C1546 (2004)
Programmed Cell Death 4 (PDCD4): A Novel Player in Ethanol Mediated Suppression of Protein Translation in Primary Cortical Neurons and Developing Cerebral Cortex
Narasimhan M, et al.
Alcoholism, Clinical and Experimental Research, 37(1), 96-96 (2013)
Cory M Howard et al.
Frontiers in oncology, 9, 284-284 (2019-05-21)
Triple-negative breast cancer (TNBC) remains clinically challenging as effective targeted therapies are lacking. In addition, patient mortality mainly results from the metastasized lesions. CXCR4 has been identified to be one of the major chemokine receptors involved in breast cancer metastasis.
Jesse J Chen et al.
The Journal of biological chemistry, 286(47), 40867-40877 (2011-10-05)
Ubiquitin-activating enzyme (UAE or E1) activates ubiquitin via an adenylate intermediate and catalyzes its transfer to a ubiquitin-conjugating enzyme (E2). MLN4924 is an adenosine sulfamate analogue that was identified as a selective, mechanism-based inhibitor of NEDD8-activating enzyme (NAE), another E1
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