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OGS590

Sigma-Aldrich

PUC19 - 高拷贝蓝/白克隆质粒

plasmid vector for molecular cloning

别名:

snapfast 载体

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

形式

buffered aqueous solution

分子量

size 2686 bp

菌种筛选

ampicillin

复制起点

pUC (500 copies)

肽切割

no cleavage

启动子

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

报告基因

none

运输

ambient

储存温度

−20°C

一般描述

这种高拷贝数质粒是通用的工业标准克隆载体,可用于使用蓝/白筛选进行插入和亚克隆基因。

应用

基因克隆:PUC19 - 高拷贝蓝/白克隆质粒是数千个实验室在常规克隆中使用的标准克隆质粒。该质粒可用于蓝白筛选,并含有多个多克隆位点,其从EcoRI延伸至Hind3限制性位点,允许插入DNA序列。

序列

欲查看本品的序列信息,请访问 产品页面

分析说明

欲查看本品的分析证书,请访问 www.oxgene.com

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Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

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