OGS558
PSF-OXB15 - STRONG PROMOTER E.COLI VECTOR
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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25 EA
CN¥513.45
CN¥513.45
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25 EA
CN¥513.45
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
CN¥513.45
请联系客服了解存货情况
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重组
expressed in E. coli
表单
buffered aqueous solution
分子量
size 3857 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
启动子
Promoter name: OXB15
Promoter activity: constitutive
Promoter type: bacterial
报告基因
none
运输
ambient
储存温度
−20°C
1 of 4
此商品 | B6555 | B6930 | B6305 |
|---|---|---|---|
| material colorless bottle, low-density polyethylene cap | material low-density polyethylene , round bottle, white bottle | material low-density polyethylene , natural bottle, round bottle | material colorless bottle, low-density polyethylene , polypropylene cap, round bottle |
| capacity 8 mL | capacity 8 mL | capacity 8 mL | capacity 15 mL |
| joint Joints threaded neck (15) | joint Joints threaded neck (15-415) | joint Joints threaded neck (15) | joint Joints threaded neck (15) |
| packaging pack of 25 ea | packaging pack of 25 ea | packaging pack of 25 ea | packaging pack of 25 ea |
| color white cap | color white cap | color assorted colors cap | color white cap |
一般描述
PSF-OXB15 - STRONG PROMOTER E.COLI VECTOR contains a constitutive promoter for protein expression in E. coli. The promoter is called OXB15 and is one of a range of bacterial promoters that we sell with different levels of expression. OXB1 is the lowest/weakest and OXB20 is the highest. This promoter exhibits intermediate levels of expression. This plasmid vector does not require induction for activity.
Promoter Expression Level: This plasmid vector contains a strong constitutive E. coli promoter that was derived from the RecA promoter by random mutagenesis. It is part of our constitutive bacterial promoter range. This promoter (OXB15) shows the high levels of expression in the range with OXB1 showing the lowest level and OXB20 showing the highest level of expression. This cloning vector require no inducing agent for expression.
Promoter Expression Level: This plasmid vector contains a strong constitutive E. coli promoter that was derived from the RecA promoter by random mutagenesis. It is part of our constitutive bacterial promoter range. This promoter (OXB15) shows the high levels of expression in the range with OXB1 showing the lowest level and OXB20 showing the highest level of expression. This cloning vector require no inducing agent for expression.
应用
Cloning in a gene: PSF-OXB15 - STRONG PROMOTER E.COLI VECTOR has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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