OGS3037
PSF-OXB20-COOH-FLUC - C-TERMINAL FLUC TAG BACTERIAL PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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1 L
CN¥671.60
CN¥671.60
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1 L
CN¥671.60
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
CN¥671.60
请联系客服了解存货情况
重组
expressed in E. coli
偶联物
firefly luciferase conjugate
表单
buffered aqueous solution
分子量
size 5501 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
肽标签位置
C-terminal
启动子
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
报告基因
firefly luciferase
一般描述
This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains a c-terminal Firefly luciferase reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
About the Peptide Tag:This plasmid contains a c-terminal Firefly luciferase reporter tag that can be fused to a gene of interest to allow protein detection.
About the Cleavage Tag: This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
序列
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
分析说明
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
其他说明
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
法律信息
Oxford Genetics is a trademark of Oxford Genetics Ltd
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价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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