OGS286
PSF-CMV-FMDV-RLUC - FMDV IRES RENILLA LUCIFERASE VECTOR
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
登录查看公司和协议定价
选择尺寸
100 μL
¥3,875.92
¥3,875.92
预计发货时间2025年7月31日详情
新价格，新优惠！
所有图片(2)
选择尺寸
变更视图
100 μL
¥3,875.92
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥3,875.92
预计发货时间2025年7月31日详情
新价格，新优惠！
推荐产品
表单
buffered aqueous solution
分子量
size 5650 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
Renilla luciferase
运输
ambient
储存温度
−20°C
1 of 4
此商品 | C8726 | C1224 | C6607 |
|---|---|---|---|
| assay ≥90% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay >90% (SDS-PAGE) |
| recombinant expressed in E. coli (C-terminal histidine tagged) | recombinant expressed in E. coli (C-terminal histidine-tagged) | recombinant expressed in E. coli (C-terminal histidine-tagged) | recombinant expressed in E. coli |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous glycerol solution | form lyophilized powder |
| Gene Information human ... CASP8(841) | Gene Information human ... CASP9(842) | Gene Information human ... CASP3(836) | Gene Information human ... CASP10(843) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
一般描述
Plasmid vector for molecular cloning allows the expression of two genes from one vector where the second gene produced from the mRNA is the Renilla reniformis luciferase reporter gene under the control of the internal ribosome entry site (IRES) from Foot and Mouth Disease virus (FMDV). Transcription is driven by the CMV promoter. There is a multiple cloning site immediately downstream of the CMV promoter which is then followed by the IRES element driving luciferase.
Promoter Expression Level: PSF-CMV-FMDV-RLUC - FMDV IRES Renilla luciferase vector contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: PSF-CMV-FMDV-RLUC - FMDV IRES Renilla luciferase vector contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
应用
Cloning in a gene: PSF-CMV-FMDV-RLUC - FMDV IRES Renilla luciferase vector has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
