OGS1428
PSF-CMV-PURO-NH2-INSULINSP-10HIS - INSULIN SECRETION AND 10HIS TAG PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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5 MG
¥1,506.88
25 MG
¥5,784.39
¥1,506.88
国内现货，预计发货时间2025年4月25日详情
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5 MG
¥1,506.88
25 MG
¥5,784.39
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥1,506.88
国内现货，预计发货时间2025年4月25日详情
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标签
10-His tagged
表单
buffered aqueous solution
分子量
size 6245 bp
菌种筛选
kanamycin
哺乳动物细胞筛选
puromycin
复制起点
pUC (500 copies)
肽切割
no cleavage
肽标签位置
N-terminal
启动子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
报告基因
none
1 of 4
此商品 | SML1094 | 361559 | C9873 |
|---|---|---|---|
| form powder | form powder | form solid | form powder |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97% (HPLC) | assay ≥98% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility DMSO: 2 mg/mL, clear (warmed) | solubility DMSO: 0.5 mg/mL, clear (warmed) | solubility DMSO: 5 mg/mL | solubility DMSO: >10 mg/mL |
| color white to light brown | color white to brown | color light beige | color white to beige |
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an Human Insulin (iSP) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. This plasmid also contains a secondary Deca-Histidine (10His) protein tag. The sequence of this tag is: HHHHHHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an Human Insulin (iSP) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. This plasmid also contains a secondary Deca-Histidine (10His) protein tag. The sequence of this tag is: HHHHHHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
分析说明
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
其他说明
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
法律信息
Oxford Genetics is a trademark of Oxford Genetics Ltd
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说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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